CONDITIONALLY REPLICATING ADENOVIRUS Russian patent published in 2018 - IPC C12N15/861 C12N15/33 C12Q1/02 C12N15/65 

FIELD: medicine.

SUBSTANCE: recombinant adenovirus is proposed for detecting cancer cells or diagnosing cancer containing a replicate cassette, a marker cassette, a gene that encodes the CD46 binding fibrillar protein. Specified replicate cassette is integrated into the part of E1 adenovirus genome and contains human telomerase reverse transcriptase promoter, E1a gene, sequence IRES and gene E1V in said order and sequence-target miR-142. The specified marker cassette is integrated into the part of adenovirus genome E3 and consists of reporter gene, promoter, able to regulate gene expression, and sequence-targets miR-142. Proposed recombinant adenovirus allows to detect cancer cells all over the body, including CAR-negative cells, wherein does not give false positive results in the detection of cancer cells to normal blood cells.

EFFECT: diagnosis of cancer.

4 cl, 8 dwg, 7 ex