VISCOSITY OF ENVIRONMENT AS TOOL TO CONTROL END OF IMMUNOCHROMATOGRAPHIC TEST SYSTEMS DETECTION Russian patent published in 2018 - IPC G01N11/00 G01N33/50 

FIELD: chemistry.

SUBSTANCE: immunochromatographic test is based on the interaction of a specific antibody-colloid marker conjugate with a detectable compound (antigen) during the movement of the reagents along the test strip. Depending on the presence of the antigen, specific complexes are formed that provide staining in the analytical test line. The intensity of the staining, as well as the sensitivity of the test, directly depend on the incubation time of the sample and the colloidal conjugate. Significant possibilities for reducing the detection limit are provided by the use of small-pored, "slow" working membranes. Reducing the speed of movement increases the time of a specific interaction and thereby reduces the detection limit of the system. However, the use of finely porous membranes often results in a number of negative effects, especially when analysing real samples, often containing corpuscular particles. In such cases, the pores of the membrane are clogged and the fluid stops flowing completely, and the test results are recognized as invalid. The proposed approach is characterized in that a large porous membrane is used for analysis, and to reduce the rate of fluid movement special solutions are added to the sample to increase the viscosity of the solution, which leads to an increase in the time along the membrane.

EFFECT: reducing the detection limit of the analytical system.

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