FIELD: biotechnology.
SUBSTANCE: group of inventions is described, comprising a pharmaceutical composition for polypeptide expression in a mammalian cell and application of a composition for preparation of a medicament to increase the level of the polypeptide of interest in a mammal. The specified polynucleotide contains: (a) an open reading frame encoding the specified polypeptide consisting of nucleosides, including 1-methyl pseudouridine, cytidine or 5-methylcytidine, adenosine, and guanosine, (b) 5'-UTR, (c) 3'-UTR, and (d) 5'-cap structure.
EFFECT: invention expands the arsenal of means for polynucleotides expressing.
6 cl, 10 dwg, 50 tbl, 90 ex
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