FIELD: biotechnology.
SUBSTANCE: following is described: a method of purifying mRNA molecules and a method of producing a pharmaceutical composition including the step of purifying mRNA using the above method of purifying mRNA molecules. The purification method includes the steps of (Ia) purifying precipitated mRNA molecules from a suspension including precipitated mRNA molecules using a first solution, (Ib) washing and dissolving the purified precipitated mRNA molecules obtained in step (Ia) using a second solution, (IIa) purifying the mRNA molecules from the dissolved mRNA molecules obtained in step (Ib) using a third solution including a chelating agent, and (IIb) washing the purified mRNA molecules obtained in step (IIa) using a fourth solution. Stages (Ia)-(IIb) are carried out using tangential flow filtration.
EFFECT: invention expands the arsenal of high-throughput methods of obtaining purified mRNA molecules.
15 cl, 12 dwg, 13 tbl, 1 ex
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2020 |
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