FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. Described is a composition for expression of a polypeptide in a mammalian cell containing an effective amount of a plurality of lipid nanoparticles containing a polynucleotide encoding said polypeptide. In one embodiment, the polynucleotide comprises (a) an open reading frame encoding said polypeptide consisting of nucleosides, including 1-methylpseudouridine, cytidine or 5-methylcytidine, adenosine and guanosine, (b) 5'-UTR, (c) 3'-UTR, and (d) 5'-cap structure.
EFFECT: invention extends the range of agents for expression of polypeptides in a mammalian cell.
8 cl, 10 dwg, 50 tbl, 90 ex
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