METHOD OF EFFECTIVE AND SAFE CRYOPRESERVATION OF DONOR VASCULAR GRAFTS, ENSURING THE OPTIMIZATION OF THEIR FURTHER PROCESSING - RADIATION STERILIZATION AND DECELLULARIZATION Russian patent published in 2018 - IPC A01N1/00 

FIELD: medicine.

SUBSTANCE: invention relates to cryopreservation of biological material, in particular vascular allografts. Proposed method of cryopreservation of vascular allografts includes ultra-rapid cooling of allografts to a temperature of -80 °C and storage at this temperature, while the allografts are attached to sterile silicone or plastic hollow substrates with a diameter of 15% less than the internal diameter of the vessels and pre-cooled to +4 °C, and for cryoconservation as a coolant and non-penetrating cryoprotector, polydimethylsiloxane (PDMS) is used with a viscosity of 1 cSt with a temperature of -80 °C. Cooling and freezing passes at a speed of -10 °C/s by immersion and displacement in the volume of PDMS for 1 min. For individual packaging of allografts, a sterile 50 ml tube with a hole at the bottom and a port in the lid and two flaps at the ends of the substrate is used on the substrates. For packaging of 3 allografts, an intermediate plastic container of 50 ml volume is used, having a lid with a port for evacuation and with three ports for substrates at the bottom, at the same distance from each other, which is installed in a vertical position in a transparent polyacrylate container of 1 volume l. For packaging of 10 allografts, two holders are used with through channels for the substrates in a horizontal position and with transparent covers to be attached to these holders. Allografts are stored in selected package coated with PDMS. Heating of allografts is carried out on substrates in a volume of PDMS with a viscosity of 25 cSt with a temperature of +25°C for 5 minutes. For safe radiation sterilization, each package with allografts is evacuated.

EFFECT: proposed method of cryopreservation of donor vascular allografts avoids formation of extracellular ice crystals, the use of cryoprotectants that have a cytotoxic effect on the graft, eliminate use of software freezing methods with the use of liquid nitrogen and ensure the optimization of their further processing - radiation sterilization and decellularization.

1 cl, 1 dwg