METHOD FOR PRODUCING A HOMOGRAFT OF THE CARDIOVASCULAR SYSTEM BY MEANS OF CRYO-CONSERVATION Russian patent published in 2022 - IPC A01N1/02 A61F2/07 

FIELD: medicine.

SUBSTANCE: invention relates to cardiac surgery and pertains to a method for creating homografts of the cardiovascular system (HCVS) by means of cryo-conservation. Method for producing a homograft of the cardiovascular system by cryo-conservation using polydimethylsiloxane as a cooling agent, cryo-protective and cryo-conserving agent, includes the stages of sampling a section of a great vessel as a biological tissue component; conducting morphometry thereof; assessing the level of initial contamination; pre-cooling by placing the extracted component in a Custodiol solution at +4°C; creating a cryo-protective layer on the surface of the component by submerging said component in a volume of polydimethylsiloxane with a viscosity of 1 cSt and a temperature of +4°C; cryo-conserving by submerging the resulting component in a volume of polydimethylsiloxane with a viscosity of 1 cSt and a temperature of -80°C while moving in the volume to ensure uniform freezing; packing the resulting homograft in a self-sealing packet; evacuating and subjecting the packet to radiation sterilisation; storing at a -80°C in a freezer; removing the homograft from the packet before use and defrosting by submerging in a volume of polydimethylsiloxane. Herewith, in addition to the section of a main vessel, a section of the outflow tract of the right or left ventricle, mitral or aortic cardiac valve, or a fragment of the aorta is used as a biological tissue component; the component is collected so as to preserve the non-functional adjacent section intended to ensure manipulations in the course of implementation of the method, without contact with the component and the finished homograft, by capturing said section with an instrument, primarily with forceps; cryo-conservation is conducted for at least 1 minute with an average freezing rate of 300°C/min with the speed of manual displacement of the component in the volume of polydimethylsiloxane of 1 to 2 rps or with the speed of automatic mixing of the solution around the component in the cryostat of 0.2 m/s; the homograft is defrosted before implantation at an average speed of 500°C/min by submerging for 1 minute in a volume of polydimethylsiloxane with a viscosity of 5 cSt at a temperature of +30°C, and after defrosting, the non-functional section is removed, wherein industrially sterilised polydimethylsiloxane or polydimethylsiloxane filtered under aseptic conditions is used for cooling, cryo-conservation, and defrosting.

EFFECT: possibility of expanding the scope of possibilities of prosthetics of the cardiovascular system, simplifying and reducing the duration of the procedure for producing HCVS.

1 cl, 2 ex