METHOD FOR CLEANING THE MEDICAMENT OF PROLONGED ACTION ON THE BASIS OF THE RECOMBINANT ANALOGUE OF ALPHA-17 INTERFERON FOR VIRAL HEPATITIS C TREATMENT Russian patent published in 2018 - IPC C07K1/18 C07K1/20 C07K1/22 C07K14/56 C07K19/00 C12N15/62 C12R1/19 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, in particular to a method for purifying recombinant interferon alpha-17 analog protein represented by the sequence of SEQ ID NO: 1. Soluble fraction of fused recombinant protein with thioredoxin stabilizer protein from Escherichia coli BL21(DE3) producing strain transfected with the pET-32a plasmid DNA carrying the gene represented by the sequence of SEQ ID NO 2 is obtained. Chromatographic purification on a metal-affinity sorbent using Ni-Sepharose is carried out. Next, hydrolysis of the fusion recombinant protein with enzyme enterokinase is carried out, incubating for 1 hour at 37 °C. Denaturation of impurities with heating to 64 °C for 60 min is carried out. FPLC is purified on the SP-Sepharose ion exchange sorbent and reverse phase HPLC purification of the target recombinant protein on Superdex 75 column are carried out.

EFFECT: invention makes it possible to significantly enrich the desired product, resulting in its homogeneity and chromatographic purity of more than 99 %.

1 cl, 15 dwg, 6 ex