METHOD FOR OBTAINING A HYBRID PROTEIN CONTAINING A FUSED PROTEIN ANALOGUE OF AN INTERFERON GAMMA CONJUGATED WITH OLIGOSACCHARIDE Russian patent published in 2018 - IPC C07K14/57 A61K38/21 

FIELD: biotechnology.

SUBSTANCE: method comprises conjugating an interferon gamma fused analogue represented by the sequence of SEQ ID NO: 1, with an oligosaccharide-type polymer D3000-7 (Sa-Sp)-(Sp-M) having the structure shown in Fig. 8, where D3000 is the dialdehyde dextran, (Sa-Sp) - Neu5Ac-Lactose-N(CH)-CH(2)-CH(2)-NH(2), (Sp-M) - Maleimide-CH(2)-NH(2), purification of the resulting conjugated interferon gamma fused analogue protein of unconjugated components by the HPLC method with the Sephacryl S400 gel filtration sorbent with the yield of the final product containing impurities not more than 1.2% of the total product mass.

EFFECT: method for producing a fusion protein based on a interferon gamma fused analogue having antiviral activity is proposed.

1 cl, 16 dwg, 2 tbl