FIELD: biotechnology.
SUBSTANCE: nucleotide sequence encoding a recombinant interferon lambda protein is optimized for expression in E. coli cells and cloned into the rET30a plasmid. E. coli BL-30-L cell, intended for interferon lambda recombinant protein production is obtained by transformation of E. coli BL (DE3) cells by rET30a plasmid containing the said nucleic acid. The invention provides a recombinant interferon lambda protein with biological activity of 2.1*109 U/mcmol in MDBK/VSV system.
EFFECT: increased biological activity.
2 cl, 4 dwg, 3 tbl, 6 ex
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