FIELD: biotechnology.
SUBSTANCE: isolated polynucleotide substrate for a catalytic nucleic acid with enzymatic activity and its use, method for detecting the presence of a target in a sample using said substrate, as well as a kit for detecting a target molecule and a kit for detecting a target molecule containing said substrate, and a kit for use in a signaling cascade comprising said kit. Proposed isolated polynucleotide substrate can be efficiently catalytically modified over a wide range of temperatures.
EFFECT: proposed group of inventions can be used in biotechnology to detect the presence of a target in a sample.
36 cl, 13 dwg, 25 tbl, 10 ex
