RNA-CONDUCTORS TO SUPPRESS THE REPLICATION OF HEPATITIS B VIRUS AND FOR THE ELIMINATION OF HEPATITIS B VIRUS FROM HOST CELL Russian patent published in 2018 - IPC C12N15/00 C12N15/11 C12N15/113 C12N15/51 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, namely, to genetic engineering. RNA-conductor (RNA guide) has been described for suppression the expression of the hepatitis B virus in a host cell and for eliminating HBV DNA from a host cell, wherein said RNA-conductor comprises a first nucleotide sequence selected from SEQ ID NO: 1 and SEQ ID NO: 2 and the second nucleotide sequence of SEQ ID NO: 5, flanking the first sequence from the 3 'end and representing an RNA-pin, and said first sequence is capable of binding to a target highly conserved region of the hepatitis B virus genome, and said second sequence contains a RAM-motive capable of being recognized by the RNA-directed Cas9 Streptococcus pyogenes DNA endonuclease, with the assurance of eliminating the hepatitis B virus. Also, an RNA transducer for suppressing the expression of the hepatitis B virus in the host cell and for eliminating HBV DNA from host cell is described, wherein said RNA-conductor comprises a first nucleotide sequence selected from SEQ ID NO: 3 and SEQ ID NO: 4, and the second nucleotide sequence of SEQ ID NO: 6, flanking the first sequence from the 3'-end and representing an RNA-hairpin, said first sequence being capable of binding to the target highly conserved region of the hepatitis B virus genome, and said second sequence is capable of being recognized by the RNA-directed Cas9 Streptococcus thermophilus DNA assay to ensure the elimination of the hepatitis B virus. Inventions can be used in CRISPR-Cas9 systems to affect highly conserved regions of the hepatitis B virus (HBV) genome, followed by elimination of HBV virus DNA, including annular covalently closed DNA (accDNA) of HBV, from a host cell, such as a mammalian cell (eg, human), in particular from human hepatocyte, as well as to suppress replication of the hepatitis B virus.

EFFECT: in comparison with prior art RNA conductors used in CRISPR-Cas9 systems to effect HBV DNA, the RNA conductors of the invention provide enhanced effect of such systems on highly conserved regions of the hepatitis B virus genome and shorten the timing of elimination of viral DNA from the host cell.

10 cl, 8 dwg, 2 tbl, 3 ex