RNA-CONDUCTOR St10 FOR USE IN THE HIGHLY SPECIFIC STREPTOCOCCUS THERMOPHILUS CRISPR / Cas9 (StCas9) NUCLEASES SYSTEM AND USE OF SAID RNA-CONDUCTOR AND StCas9 PROTEIN TO SUPPRESS HEPATITIS B VIRUS EXPRESSION IN THE HOST CELL AND FOR VIRAL DNA ELIMINATION FROM THE HOST CELL Russian patent published in 2019 - IPC C12N15/00 C12N15/11 C12N15/113 C12N15/51 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, biotechnology and genetic engineering. What is described is a complex of StCas9 protein and a new RNA-conductor for suppression of hepatitis B virus expression in a host cell and for elimination of hepatitis B virus DNA from a host cell, such as a mammalian cell. Also described is use of StCas9 protein combined with RNA-conductor to suppress hepatitis B virus expression in a host cell and for DNA degradation of hepatitis B virus and its elimination from a host cell, including annular covalently closed DNA, from said host cell. Said RNA-conductor is formed by the first nucleotide sequence SEQ ID NO: 1 (AGAAAGGCCTTGTAAGTTGG) and a second nucleotide sequence, which is an RNA-pin flanking said first sequence from 3'-end and characterized by a sequence SEQ ID NO: 2 (AACGCGGUCGCCAAAGAGAUGUUUUUGUACUCUGGUACCAGAAGCUACAAAGAUAAGGCUUCAUGCCGAAAUCAACA CCCUGUCAUUUUAUGGCAGGGUGUUUU) and structure, presented in fig. 1. Said first sequence is able to bind to the target highly conserved portion of hepatitis B virus DNA. Said second sequence is capable of attracting the protein-endonuclease Cas9 Streptococcus thermophilus (StCas9) to said target highly conserved portion of viral DNA with ensuring the degradation of said hepatitis B virus DNA by said StCas9 endonuclease protein without the formation of off-target gaps in the human genome.

EFFECT: invention provides a new agent for influencing DNA of hepatitis B virus – RNA-conductor St10 in complex with StCas9 protein, allowing to affect DNA of hepatitis B virus, wherein target of said RNA-conductor is conservative in many clinically significant genotypes of hepatitis B virus (genotypes A, B, C, D, E and H), thereby providing degradation of said DNA and its elimination from host cell.

20 cl, 9 dwg, 4 tbl