FIELD: biotechnology.
SUBSTANCE: invention relates to cellular biology and biotechnology, specifically to the creation of a bioresorbable cellular scaffold based on fibrin of the blood plasma. Method comprises mixing a blood plasma or a cryoprecipitate of a blood plasma with a solution of type I collagen having pH of 7.4, introducing a suspension of animal cells and adding a thrombin-calcium mixture in a ratio of 3.94:2.86:1:1.09, respectively.
EFFECT: invention allows to obtain a bioresorbable cellular scaffold having a 3D structure and containing cells uniformly distributed throughout the thickness.
1 cl, 6 dwg, 3 ex
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Authors
Dates
2018-05-08—Published
2017-04-11—Filed