FIELD: biotechnology.
SUBSTANCE: invention relates to recombinant proteins containing antigenic epitopes of proteins Core, Small Envelope, NS2A and preM of the Zika virus, and a complex antigen containing these proteins. Obtained: recombinant protein (Z1), containing the antigenic epitopes of the Core protein of the Zika virus, characterized by the amino acid sequence (SEQ ID NO: 1) and used as the first component for the preparation of the complex antigen of the Zika virus; recombinant protein (Z2) containing the antigenic epitopes of the Small Envelope protein of the Zika virus, characterized by an amino acid sequence (SEQ ID NO: 2) and used as a second component to obtain the complex antigen of the Zika virus. Recombinant protein (Z3) containing the antigenic epitopes of the NS2A proteins of the Zika virus, characterized by an amino acid sequence (SEQ ID NO: 3), is used as the third component for the preparation of the complex antigen of the Zika virus, and the recombinant protein (Z4) containing the antigenic epitopes of the preM protein of the Zika virus, characterized by the amino acid sequence (SEQ ID NO: 4), it is used as the fourth component for the preparation of the complex antigen of the Zika virus. Complex antigen can be used as a component of an enzyme-linked immunosorbent assay system for the detection of G and M antibodies to the Zika virus and contains recombinant proteins Z1, Z2, Z3, Z4, mixed respectively in a ratio of 1:3:2:2. In the resulting and used proteins, there are no linear antigenic epitopes of other flaviviruses, and the sequences encoding these proteins do not have an antigenic cross with all known flaviviruses, which allows them to use more sensitive and accurate ELISA analysis.
EFFECT: invention can be used in medical virology.
5 cl, 4 tbl, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PRODUCING ANTIGEN OF ZIKA VIRUS, HAVING IMMUNOGENIC AND ANTIGENIC PROPERTIES | 2019 |
|
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|
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|
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| IMMUNOBIOLOGICAL AGENT FOR THE PREVENTION OF DISEASES CAUSED BY TICK-BORNE ENCEPHALITIS VIRUS BASED ON A RECOMBINANT VIRUS OF THE GENUS FLAVIVIRUS | 2022 |
|
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| RECOMBINANT PLASMID pHis6-flagG-protE, WHICH PROVIDES SYNTHESIS OF A RECOMBINANT CHIMERIC PROTEIN, INCLUDING EPITOPES OF GLYCOPROTEIN E OF TICK-BORNE ENCEPHALITIS VIRUS AND FLAGELLIN G SALMONELLA TYPHII AND USED AS A BASE FOR VACCINE AGAINST TICK-BORNE ENCEPHALITIS VIRUS | 2018 |
|
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| SET OF ANTIGENS FOR ASSAY OF ANTIBODIES RAISED AGAINST HEPATITIS C VIRUS "DS-HCV-ANTIGENS" | 2004 |
|
RU2262704C1 |
| STRAIN OF HYBRID ANIMAL CELLS Mus musculus L. 5H6 - PRODUCER OF MONOCLONAL ANTIBODIES FOR DETECTING GLYCOPROTEIN E OF WEST NILE VIRUS, 5H6 MONOCLONAL ANTIBODIES PRODUCED BY SAID STRAIN OF HYBRID CELLS, AND IMMUNOENZYMOMETRIC KIT FOR DETECTING GLYCOPROTEIN E OF WEST NILE VIRUS USING SAID MONOCLONAL ANTIBODIES | 2015 |
|
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| RECOMBINANT PLASMID DNA pET160-E3-YFV EXPRESSING AN ANALOGUE OF THE THIRD DOMAIN OF THE SURFACE PROTEIN E OF THE YELLOW FEVER VIRUS (YFV) IN THE BACTERIAL SYSTEM OF E. COLI USED TO DETECT SPECIFIC ANTIBODIES OF YFV | 2022 |
|
RU2798563C1 |
| mRNA-BASED IMMUNOBIOLOGICAL AGENT FOR PREVENTION OF DISEASES CAUSED BY TICK-BORNE ENCEPHALITIS VIRUS | 2024 |
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