RECOMBINANT PROTEINS CONTAINING ANTIGENIC EPITOPES OF PROTEINS CORE, SMALL ENVELOPE, NS2A AND PREM OF ZIKA VIRUS, AND COMPLEX ANTIGEN CONTAINING SPECIFIED PROTEINS WHICH ARE USED AS A COMPONENT OF THE IMMUNOENZYMOMETRIC TEST SYSTEM FOR THE IDENTIFICATION OF ANTIBODIES CLASSES G AND M TO ZIKA VIRUS Russian patent published in 2018 - IPC C07K14/18 A61K39/12 

Abstract RU 2661085 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to recombinant proteins containing antigenic epitopes of proteins Core, Small Envelope, NS2A and preM of the Zika virus, and a complex antigen containing these proteins. Obtained: recombinant protein (Z1), containing the antigenic epitopes of the Core protein of the Zika virus, characterized by the amino acid sequence (SEQ ID NO: 1) and used as the first component for the preparation of the complex antigen of the Zika virus; recombinant protein (Z2) containing the antigenic epitopes of the Small Envelope protein of the Zika virus, characterized by an amino acid sequence (SEQ ID NO: 2) and used as a second component to obtain the complex antigen of the Zika virus. Recombinant protein (Z3) containing the antigenic epitopes of the NS2A proteins of the Zika virus, characterized by an amino acid sequence (SEQ ID NO: 3), is used as the third component for the preparation of the complex antigen of the Zika virus, and the recombinant protein (Z4) containing the antigenic epitopes of the preM protein of the Zika virus, characterized by the amino acid sequence (SEQ ID NO: 4), it is used as the fourth component for the preparation of the complex antigen of the Zika virus. Complex antigen can be used as a component of an enzyme-linked immunosorbent assay system for the detection of G and M antibodies to the Zika virus and contains recombinant proteins Z1, Z2, Z3, Z4, mixed respectively in a ratio of 1:3:2:2. In the resulting and used proteins, there are no linear antigenic epitopes of other flaviviruses, and the sequences encoding these proteins do not have an antigenic cross with all known flaviviruses, which allows them to use more sensitive and accurate ELISA analysis.

EFFECT: invention can be used in medical virology.

5 cl, 4 tbl, 6 ex

Similar patents RU2661085C1

Title Year Author Number
METHOD OF PRODUCING ANTIGEN OF ZIKA VIRUS, HAVING IMMUNOGENIC AND ANTIGENIC PROPERTIES 2019
  • Zajkovskaya Anna Vladimirovna
  • Oskina Oksana Petrovna
  • Zolin Vladimir Viktorovich
  • Pyankov Oleg Viktorovich
  • Kazachinskaya Elena Ivanovna
RU2717993C1
PVEAL3-10H10CH PLASMID GENETIC CONSTRUCT, STRAIN OF CHO-K1-10H10CH RECOMBINANT CELL LINE AND 10H10CH CHIMERIC ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS PRODUCED BY THE INDICATED STRAIN OF CHO-K1-10H10CH CELL LINE 2022
  • Shanshin Daniil Vasilevich
  • Nesmeyanova Valentina Sergeevna
  • Shcherbakov Dmitrij Nikolaevich
  • Loktev Valerij Borisovich
  • Protopopova Elena Viktorovna
  • Ilichev Aleksandr Alekseevich
RU2800471C1
TETRAVALENT VECTOR VACCINE AND METHOD OF ITS USE FOR PREVENTION OF DENGUE FEVER 2023
  • Yudin Sergej Mikhajlovich
  • Bogoslovskaya Elena Vladimirovna
  • Glazkova Dina Viktorovna
  • Mintaev Ramil Rafailovich
  • Orlova Olga Vladimirovna
  • Shipulin German Aleksandrovich
  • Skvortsova Veronika Igorevna
RU2812251C1
METHOD OF PREPARING IMMUNOGENIC COMPOSITION BASED ON THREE FUSION PROTEINS OF SHELL OF TICK-BORNE ENCEPHALITIS VIRUS DEFINING BELONGING TO SIBERIAN (DBD2-D3S), EUROPEAN (DBD2-D3E) AND FAR EAST (DBD2-D3D) VIRUS SUBTYPES; RECOMBINANT PLASMIDS pDBD2-D3S, pDBD2-D3E AND pDBD2-D3D; PRODUCER STRAINS Escherichia coli M15 [pREP4]; CHIMERIC PROTEINS AND THEIR APPLICATION 2014
  • Ljashchuk Aleksandr Mikhajlovich
  • Aksenova Ekaterina Ivanovna
  • Ershova Anna Stepanovna
  • Grunina Tat'Jana Mikhajlovna
  • Bartov Mikhail Sergeevich
  • Savina Dar'Ja Mikhajlovna
  • Manukhina Marija Sergeevna
  • Gudov Vladimir Petrovich
  • Semikhin Aleksandr Sergeevich
  • Gra Ol'Ga Alekseevna
  • Tkachuk Artem Petrovich
  • Galushkina Zoja Mikhajlovna
  • Karjagina-Zhulina Anna Stanislavovna
  • Lunin Vladimir Glebovich
  • Gintsburg Aleksandr Leonidovich
RU2560588C1
IMMUNOBIOLOGICAL AGENT FOR THE PREVENTION OF DISEASES CAUSED BY TICK-BORNE ENCEPHALITIS VIRUS BASED ON A RECOMBINANT VIRUS OF THE GENUS FLAVIVIRUS 2022
  • Kuznetsova Nadezhda Anatolevna
  • Siniavin Andrei Eduardovich
  • Usachev Evgenii Valerevich
  • Pochtovyi Andrei Andreevich
  • Nikiforova Mariia Andreevna
  • Shchetinin Aleksei Mikhailovich
  • Shidlovskaia Elena Vladimirovna
  • Divisenko Elizaveta Vladimirovna
  • Vasilchenko Liudmila Aleksandrovna
  • Zolotar Anastasiia Nikolaevna
  • Butenko Aleksandr Mikhailovich
  • Larichev Viktor Filippovich
  • Zakharova Anastasiia Andreevna
  • Remizov Timofei Andreevich
  • Rubalskii Oleg Vasilevich
  • Tokarskaia Elizaveta Aleksandrovna
  • Tkachuk Artem Petrovich
  • Gushchin Vladimir Alekseevich
  • Logunov Denis Iurevich
  • Gintsburg Aleksandr Leonidovich
RU2795800C1
RECOMBINANT PLASMID pHis6-flagG-protE, WHICH PROVIDES SYNTHESIS OF A RECOMBINANT CHIMERIC PROTEIN, INCLUDING EPITOPES OF GLYCOPROTEIN E OF TICK-BORNE ENCEPHALITIS VIRUS AND FLAGELLIN G SALMONELLA TYPHII AND USED AS A BASE FOR VACCINE AGAINST TICK-BORNE ENCEPHALITIS VIRUS 2018
  • Belavin Pavel Aleksandrovich
  • Dejneko Elena Viktorovna
  • Protopopova Elena Viktorovna
  • Konovalova Svetlana Nikolaevna
  • Loktev Valerij Borisovich
RU2702716C2
SET OF ANTIGENS FOR ASSAY OF ANTIBODIES RAISED AGAINST HEPATITIS C VIRUS "DS-HCV-ANTIGENS" 2004
  • Burkov A.N.
  • Obrjadina A.P.
  • Ulanova T.I.
  • Gladysheva M.V.
RU2262704C1
STRAIN OF HYBRID ANIMAL CELLS Mus musculus L. 5H6 - PRODUCER OF MONOCLONAL ANTIBODIES FOR DETECTING GLYCOPROTEIN E OF WEST NILE VIRUS, 5H6 MONOCLONAL ANTIBODIES PRODUCED BY SAID STRAIN OF HYBRID CELLS, AND IMMUNOENZYMOMETRIC KIT FOR DETECTING GLYCOPROTEIN E OF WEST NILE VIRUS USING SAID MONOCLONAL ANTIBODIES 2015
  • Kazachinskaya Elena Ivanovna
  • Razumov Ivan Alekseevich
  • Loktev Valerij Borisovich
RU2595429C1
RECOMBINANT PLASMID DNA pET160-E3-YFV EXPRESSING AN ANALOGUE OF THE THIRD DOMAIN OF THE SURFACE PROTEIN E OF THE YELLOW FEVER VIRUS (YFV) IN THE BACTERIAL SYSTEM OF E. COLI USED TO DETECT SPECIFIC ANTIBODIES OF YFV 2022
  • Krivosheina Ekaterina Ilinichna
  • Kartashov Mikhail Yurevich
  • Ushkalenko Nikita Dmitrievich
  • Ternovoj Vladimir Aleksandrovich
RU2798563C1
HSOBIENS-WT/GIN/2015/KALIDIE-KINDIA-1022 STRAIN OF EBOLA ZAIRE TO OBTAIN ANTIGEN USED AS COMPONENT OF IMMUNOENZYMOMETRIC TEST SYSTEM FOR DETECTION OF G AND M ANTIBODIES TO VIRUS OF EBOLA 2016
  • Pyankov Stepan Aleksandrovich
  • Pyankov Oleg Viktorovich
  • Zajkovskaya Anna Vladimirovna
  • Solodkij Vladislav Valerevich
  • Bodnev Sergej Aleksandrovich
  • Bulychev Leonid Egorovich
  • Nesterov Andrej Egorovich
  • Sergeev Artemij Aleksandrovich
  • Sergeev Aleksandr Aleksandrovich
  • Agafonov Aleksandr Petrovich
  • Mikheev Valerij Nikolaevich
RU2631937C1

RU 2 661 085 C1

Authors

Pyankov Stepan Aleksandrovich

Agafonov Aleksandr Petrovich

Ruzhkova Alesya Aleksandrovna

Maksimov Nikolaj Lvovich

Poteeva Elena Ilinichna

Zhernovaya Larisa Dmitrievna

Maksyutov Rinat Amirovich

Dates

2018-07-11Published

2017-06-01Filed