FIELD: biotechnologies.
SUBSTANCE: invention relates to the biotechnology. Described is a recombinant plasmid pHis6-flagG-protE with built-in fliG and TBEVgp1 genes coding hybrid recombinant flagG-protE protein inducing an immune response to tick-borne encephalitis virus.
EFFECT: invention provides production of recombinant chimeric protein without inclusion bodies formation, provides synthesis of recombinant chimeric protein having amino acid sequence (SEQ ID NO: 2), including epitopes of glycoprotein E of tick-borne encephalitis virus and flagellin G Salmonella typhii and used as a base for vaccine against tick-borne encephalitis virus.
1 cl, 5 dwg, 2 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT DNA PLASMID PGSDE1 ENCODING TICK-BORNE ENCEPHALITIS VIRUS PROTEIN E AND STRAIN OF ESCHERICHIA COLI - PRODUCER OF TICK-BORNE ENCEPHALITIS VIRUS PROTEIN E | 1997 |
|
RU2136754C1 |
| RECOMBINANT PLASMID DNA pSC13D6 CONTAINING GENE OF ONE-CHAINED ANTIBODY TO TICK-BORNE ENCEPHALITIS VIRUS, AND STRAIN OF BACTERIA Escherichia coli BL21 (DE3)/pSC13D6-PRODUCER OF ONE-CHAINED ANTIBODIES TO TICK-BORNE ENCEPHALITIS VIRUS, EXPRESSING VIRUS NEUTRALISING PROPERTIES | 2008 |
|
RU2378378C1 |
| RECOMBINANT PLASMID DNA PTLS CONTAINING GENE OF SINGLE-STRANDED ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS AND STRAIN OF BACTERIUM ESCHERICHIA COLI AS PRODUCER OF SINGLE-STRANDED ANTIBODIES AGAINST TICK-BORNE ENCEPHALITIS VIRUS | 1998 |
|
RU2158309C2 |
| RECOMBINANT PLASMID DNA P7SHSHIS FOR PRODUCTION OF DIMERIC SINGLE-STRANDED ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS AND STRAIN OF ESCHERICHIA COLI AS PRODUCER OF DIMERIC FORM OF SINGLE-STRANDED ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS | 2000 |
|
RU2190017C2 |
| IMMUNOBIOLOGICAL AGENT FOR THE PREVENTION OF DISEASES CAUSED BY TICK-BORNE ENCEPHALITIS VIRUS BASED ON A RECOMBINANT VIRUS OF THE GENUS FLAVIVIRUS | 2022 |
|
RU2795800C1 |
| RECOMBINANT PLASMID DNA PC-NS3 PROVIDING INTEGRATION OF COMPLEX OF GENES C, PRM, E, NS1, NS 2A, NS 2B, NS3 OF TICK-BORNE ENCEPHALITIS VIRUS IN GENOME OF BOVINE VACCINE VIRUS AND RECOMBINANT STRAIN OF BOVINE VACCINE VIRUS EXPRESSING COMPLEX OF GENES C, PRM, E, NS1, NS 2A, NS2B, NS3 OF TICK-BORNE ENCEPHALITIS VIRUS IN IMMUNIZED ORGANISM CELLS | 1995 |
|
RU2112038C1 |
| RECOMBINANT PLASMID DNA pCLm4/hygro-14D5 CODING POLYPEPTIDE WITH LIGHT CHAIN PROPERTIES OF CHIMERIC ANTIBODY TO TICK-BORNE ENCEPHALITIS VIRUS, AND RECOMBINANT PLASMID DNA pCHm2-14D5 CODING POLYPEPTIDE WITH HEAVY CHAIN PROPERTIES OF CHIMERIC ANTIBODY TO TICK-BORNE ENCEPHALITIS VIRUS, CHIMERIC ANTIBODY PROVIDING ACUTE PREVENTION OF TICK-BORNE ENCEPHALITIS IN MICE | 2013 |
|
RU2550252C1 |
| mRNA-BASED IMMUNOBIOLOGICAL AGENT FOR PREVENTION OF DISEASES CAUSED BY TICK-BORNE ENCEPHALITIS VIRUS | 2024 |
|
RU2823754C1 |
| RECOMBINANT BACTERIAL PLASMID EXPRESSING PATHOGEN PROTEIN AND BIOLOGICALLY ACTIVE FRAGMENT OF ORNITHINE DECARBOXYLASE IN MAMMAL CELLS FOR DEVELOPING AGENT FOR PREVENTION OF VIRAL INFECTIOUS DISEASES (BY EXAMPLE OF TICK-BORNE ENCEPHALITIS VIRUS) | 2009 |
|
RU2425881C1 |
| DNA-CONSTRUCT OF VIABLE CHIMERIC RECOMBINANT FLAVIVIRUS AGAINST TICK-BORNE ENCEPHALITIS VIRUS, METHOD OF PROPHYLAXIS OF INFECTION MAMMAL WITH TICK-BORNE ENCEPHALITIS VIRUS | 1998 |
|
RU2208635C2 |
