METHOD FOR PREDICTING THE DEVELOPMENT OF METASTASES IN REGIONAL LYMPH NODES IN PATIENTS WITH GASTRIC ADENOCARCINOMA Russian patent published in 2018 - IPC G01N33/48 G01N33/50 

FIELD: medicine; biotechnology.

SUBSTANCE: invention relates to molecular oncology and is a method for predicting metastases in regional lymph nodes in gastric adenocarcinoma, characterized in that amplification of fragments of loci B2M, NFKB1 and HER2 NFKB1 and HER2 by real-time PCR method, the relative abundance of genes is calculated using a formula rC=rC_tumor/rC_norm=2^-ΔCt_(tumor/2^-ΔCt_(norm), where rC is the relative abundance of the gene, ΔCt is the difference in the mean value of the fluorescence signals (C_t) by three replicates for the target gene and the mean C_t for three replicates for the reference gene: Δ_Ct=_Ct(target gene)-C_t(B2M), immunohistochemical examination is performed on sections of paraffin blocks using monoclonal antibodies to Ki67 and HER2, and at values rC_NFKB1<0.8±0.02 and rC_HER2<1.1±0.08 in combination with the level of expression in the nuclei of tumor cells of the Ki67_IHC protein <35.6±6.1 and negative expression of HER2_IHC(-), predict the absence of metastases, but at values rC_NFKB1>0.9±0.01 and rC_HER2>2.0±0.1 in combination with the level of expression in the nuclei of tumor cells of the Ki67_IHC protein >54.2±4.9 and positive expression of HER2_IHC(+++), predict the development of metastases.

EFFECT: invention provides an expansion of the arsenal of means for predicting the development of metastases, as well as the creation of a method that is fast in execution and highly sensitive.

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