FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology, in particular to the set of diaminobenzidine (DAB) -containing substrate for staining using the antibody, which is labeled with peroxidase. Set contains the chromophore-containing solution (A), which comprises 0.1 to 10 mg/ml of DAB as the peroxidase chromophore and water, and a chromogenic reagent (B) containing from 0.1 to 10 mM of at least one chelating agent, from 0.02 to 30 % by weight of a non-ionic surfactant made of polyoxyethylene alkyl ether (POE), from 3 to 300 mM imidazole, from 0.001 to 0.3 % by weight of hydrogen peroxide, and water. Moreover, the content of these components is indicated in terms of the concentration in the solution of the DAB-containing substrate for use prepared by mixing or mixing and diluting the component solutions of the kit. Chelating agent is selected from disodium salt dihydrate ethylenediaminetetraacetic acid, diammonium salt of ethylenediaminetetraacetic acid, glycolic ester of diaminetetraacetic acid and nitrile triacetic acid. Set is in the system of two or more components, at least the chromophore-containing solution (A) and the chromogenic reagent (B) are stored separately.
EFFECT: invention allows to increase the intensity of the specific staining and to reduce the precipitation due to aggregation of DAB in a solution of the DAB substrate and non-specific staining, such as background staining, in a chromogenic reaction using an antibody.
9 cl, 7 tbl, 20 ex
