FIELD: biotechnology.
SUBSTANCE: group of inventions relates to biotechnology. Proposed is a plasmid DNA pQE30-A2.1, which ensures expression of alkaline serine proteinase, comprises a nucleotide sequence encoding a S1 family recombinant alkaline serine proteinase from Streptomyces avermitilis VKM Ac-1301. Proposed is a strain E. coli M15 (pRep4, pQE30-A2.1) – producer of S1 family alkaline serine proteinase, obtained by transforming the E. coli strain M15 (pRep4) by said plasmid. Group of inventions allows to obtain previously uncharacterized proteinase from Streptomyces avermitilis VKM Ac-1301 with a molecular mass of 44.92 kDa.
EFFECT: preparation of proteinase from Streptomyces avermitilis VKM Ac-1301, which can later be used in detergents.
2 cl, 1 dwg, 1 ex
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