METHOD OF SPECIFIC IDENTIFICATION OF DNA SEQUENCES Russian patent published in 2018 - IPC G01N33/58 C12Q1/68 

Abstract RU 2665631 C2

FIELD: medicine.

SUBSTANCE: presented group of inventions refers to the field of molecular biology and medicine. Method and set are proposed for specifically identifying at least one DNA sequence in sample by carrying out polymerase chain reaction (PCR), comprising one pair of primers specific for this DNA sequence and pair of probes in which one is oligonucleotide target-specific probe, having 3'-segment complementary to the identifiable area of DNA sequence and containing label, and 5'-segment, non-complementary identifiable DNA sequence and containing label, and the other an oligonucleotide signal probe, complementary 5'-segment target-specific probe and containing label.

EFFECT: presented group of inventions allows specific detection of DNA sequences with high sensitivity and specificity.

28 cl, 6 dwg, 2 ex

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RU 2 665 631 C2

Authors

Vedernikov Vitalij Evgenevich

Dates

2018-09-03Published

2017-01-25Filed