FIELD: medicine.
SUBSTANCE: presented group of inventions refers to the field of molecular biology and medicine. Method and set are proposed for specifically identifying at least one DNA sequence in sample by carrying out polymerase chain reaction (PCR), comprising one pair of primers specific for this DNA sequence and pair of probes in which one is oligonucleotide target-specific probe, having 3'-segment complementary to the identifiable area of DNA sequence and containing label, and 5'-segment, non-complementary identifiable DNA sequence and containing label, and the other an oligonucleotide signal probe, complementary 5'-segment target-specific probe and containing label.
EFFECT: presented group of inventions allows specific detection of DNA sequences with high sensitivity and specificity.
28 cl, 6 dwg, 2 ex
Authors
Dates
2018-09-03—Published
2017-01-25—Filed