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IPC

A61K39/135(2006-01-01)

C12Q1/68(2006-01-01)

G01N33/53(2006-01-01)

G01N33/58(2006-01-01)

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Application

2017145889, 2017-12-25

(24)

Start date

2017-12-25

(22)

Actual filing date

2017-12-25

(45)

Published

2018-12-04

(72)

Inventor

Lozovoj Dmitrij AnatolevichMikhalishin Dmitrij ValerevichDoronin Maksim IgorevichStarikov Vyacheslav AlekseevichMedvedeva Nadezhda NikolaevnaShcherbakov Aleksej VladimirovichTimina Anna MikhajlovnaBorisov Aleksej Valerevich

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Uchrezhdenie Tsentr Okhrany Zdorovya Zhivotnykh"

METHOD FOR DETERMINING THE INFECTIVITY TITER OF THE FMD VIRUS IN NON-INACTIVATED RAW MATERIALS FOR VACCINES USING THE METHOD OF REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION IN REAL TIME Russian patent published in 2018 - IPC A61K39/135 C12Q1/68 G01N33/53 G01N33/58

Abstract RU 2674076 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology and the production of foot-mouth disease (FMD) vaccines, and in particular to a method for determining the infectivity titer of the FMD virus in non-inactivated raw materials for the production of vaccines using the method of RT-PCR-RT (Reverse Transcription PCR in Real Time) and developed a regression model of the dependence of the infectivity titer of FMD virus on the magnitude of the amplification threshold cycle. Based on the amplification threshold cycle value determined by the fluorescent PCR detector (C_t) using the developed negative regression model, calculate the value of the titer of FMD virus (T_WY) of different types.

EFFECT: invention allows to quickly and with a high degree of reliability determine the value of the infectivity titer of FMD virus in non-inactivated raw materials for vaccine.

4 cl, 7 tbl, 1 dwg, 6 ex

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RU 2 674 076 C1

Authors

Lozovoj Dmitrij Anatolevich

Mikhalishin Dmitrij Valerevich

Doronin Maksim Igorevich

Starikov Vyacheslav Alekseevich

Medvedeva Nadezhda Nikolaevna

Shcherbakov Aleksej Vladimirovich

Timina Anna Mikhajlovna

Borisov Aleksej Valerevich

Dates

2018-12-04—Published

2017-12-25—Filed