FIELD: biotechnology.
SUBSTANCE: described is a method for indirect determination of the titre of infectious activity of the causative agent of infectious feline rhinotracheitis in a raw material for a vaccine using a second-order differential of the point of intersection of the logistic curve of the amplification reaction of the UL35 gene region, which involves the use of the developed oligonucleotides.
EFFECT: developed method makes it possible to increase specificity of analysis, increase sensitivity and specificity of analysis, and also increase reliability of performed analysis.
2 cl, 2 dwg, 3 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR INDIRECT DETERMINATION OF TITRE OF INFECTIOUS ACTIVITY OF THE CAUSATIVE AGENT OF INFECTIOUS FELINE RHINOTRACHEITIS IN NON-INACTIVATED RAW MATERIAL FOR CULTURAL VACCINES USING THE AMPLIFICATION REACTION OF THE TARGET REGION OF THE VIRAL DNA UL26_5-GENE | 2024 |
|
RU2834248C1 |
| METHOD OF INDIRECT DETERMINATION OF TITER OF INFECTIOUS ACTIVITY OF CAUSATIVE AGENT OF INFECTIOUS FELINE RHINOTRACHEITIS FHV IN NON-INACTIVATED RAW MATERIALS FOR VACCINES USING POLYMERASE CHAIN REACTION AND QUANTITATIVE HYBRIDIZATION-FLUORESCENCE DETECTION IN REAL TIME | 2023 |
|
RU2808641C1 |
| METHOD FOR INDIRECT DETERMINATION OF TITRE OF INFECTIOUS ACTIVITY OF VACCINE STRAIN "LAVR" OF INFECTIOUS FELINE RHINOTRACHEITIS CAUSATIVE AGENT IN RAW MATERIAL FOR VACCINE BASED ON ANALYSIS OF MELTING CURVES OF HIGH-RESOLUTION AMPLICONS OF GENE UL47 USING DSSAFE FLUORESCENT DYE | 2024 |
|
RU2834233C1 |
| METHOD FOR INDIRECT DETERMINATION OF FELINE CALICIVIRUS INFECTIOUS ACTIVITY TITRE IN NON-INACTIVATED RAW MATERIAL FOR CULTURAL VACCINES BY MEANS OF QUANTITATIVE REGISTRATION OF REACTION OF AMPLIFICATION OF ORF1-GENE SECTION OF VIRAL RNA | 2023 |
|
RU2821027C1 |
| METHOD FOR INDIRECTLY DETERMINING THE TITER OF THE INFECTIOUS ACTIVITY OF FMDV IN NON-INACTIVATED RAW MATERIALS FOR VACCINES USING A MATHEMATICAL DOUBLE DIFFERENTIAL OF THE CROSSING POINT DATA DURING AMPLIFICATION OF VIRAL CDNA | 2022 |
|
RU2793900C1 |
| METHOD OF INDIRECT DETERMINATION OF TITER OF INFECTIOUS ACTIVITY OF CANINE MASTADENOVIRUS IN RAW MATERIALS FOR VACCINES USING REAL-TIME PCR | 2023 |
|
RU2812858C1 |
| METHOD FOR DETERMINING TITRE OF INFECTIOUS ACTIVITY OF CANINE DISTEMPER AGENT IN RAW MATERIAL FOR MAKING CULTURE INACTIVATED VACCINES | 2024 |
|
RU2831062C1 |
| METHOD FOR INDIRECT DETERMINING OF THE TITER OF INFECTIOUS ACTIVITY OF FELINE CALICIVIRUS IN RAW MATERIALS FOR VACCINE USING REAL-TIME PCR | 2023 |
|
RU2809221C1 |
| METHOD OF INDIRECT DETERMINING TITER OF INFECTIOUS ACTIVITY OF RABIES VIRUS IN RAW MATERIALS FOR PRODUCTION OF ATTENUATED RABIES VACCINE USING TECHNOLOGY OF ALGEBRAIC ANALYSIS OF SECOND-ORDER DIFFERENTIAL OF MAXIMUM POINT d CPmax OF PCR LOGISTIC CURVE | 2023 |
|
RU2811995C1 |
| METHOD FOR DIFFERENTIATION OF LAVR VACCINE STRAIN OF INFECTIOUS RHINOTRACHEITIS AGENT FROM OTHER STRAINS AND FIELD ISOLATES OF FELINE INFECTIOUS RHINOTRACHEITIS VIRUS BY ANALYSING MELTING TEMPERATURE CURVE OF PCR V1-GENE PRODUCTS USING ORANGE POLYMER DYE SUPERNOVA V605 | 2024 |
|
RU2824661C1 |
