FIELD: biotechnology.
SUBSTANCE: described is a method for indirect determination of the titre of infectious activity of the causative agent of infectious feline rhinotracheitis in a raw material for a vaccine using a second-order differential of the point of intersection of the logistic curve of the amplification reaction of the UL35 gene region, which involves the use of the developed oligonucleotides.
EFFECT: developed method makes it possible to increase specificity of analysis, increase sensitivity and specificity of analysis, and also increase reliability of performed analysis.
2 cl, 2 dwg, 3 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF INDIRECT DETERMINATION OF TITER OF INFECTIOUS ACTIVITY OF CAUSATIVE AGENT OF INFECTIOUS FELINE RHINOTRACHEITIS FHV IN NON-INACTIVATED RAW MATERIALS FOR VACCINES USING POLYMERASE CHAIN REACTION AND QUANTITATIVE HYBRIDIZATION-FLUORESCENCE DETECTION IN REAL TIME | 2023 |
|
RU2808641C1 |
| METHOD FOR INDIRECT DETERMINATION OF FELINE CALICIVIRUS INFECTIOUS ACTIVITY TITRE IN NON-INACTIVATED RAW MATERIAL FOR CULTURAL VACCINES BY MEANS OF QUANTITATIVE REGISTRATION OF REACTION OF AMPLIFICATION OF ORF1-GENE SECTION OF VIRAL RNA | 2023 |
|
RU2821027C1 |
| METHOD FOR INDIRECTLY DETERMINING THE TITER OF THE INFECTIOUS ACTIVITY OF FMDV IN NON-INACTIVATED RAW MATERIALS FOR VACCINES USING A MATHEMATICAL DOUBLE DIFFERENTIAL OF THE CROSSING POINT DATA DURING AMPLIFICATION OF VIRAL CDNA | 2022 |
|
RU2793900C1 |
| METHOD OF INDIRECT DETERMINATION OF TITER OF INFECTIOUS ACTIVITY OF CANINE MASTADENOVIRUS IN RAW MATERIALS FOR VACCINES USING REAL-TIME PCR | 2023 |
|
RU2812858C1 |
| METHOD FOR DETERMINING TITRE OF INFECTIOUS ACTIVITY OF CANINE DISTEMPER AGENT IN RAW MATERIAL FOR MAKING CULTURE INACTIVATED VACCINES | 2024 |
|
RU2831062C1 |
| METHOD FOR INDIRECT DETERMINING OF THE TITER OF INFECTIOUS ACTIVITY OF FELINE CALICIVIRUS IN RAW MATERIALS FOR VACCINE USING REAL-TIME PCR | 2023 |
|
RU2809221C1 |
| METHOD OF INDIRECT DETERMINING TITER OF INFECTIOUS ACTIVITY OF RABIES VIRUS IN RAW MATERIALS FOR PRODUCTION OF ATTENUATED RABIES VACCINE USING TECHNOLOGY OF ALGEBRAIC ANALYSIS OF SECOND-ORDER DIFFERENTIAL OF MAXIMUM POINT d CPmax OF PCR LOGISTIC CURVE | 2023 |
|
RU2811995C1 |
| METHOD FOR DIFFERENTIATION OF LAVR VACCINE STRAIN OF INFECTIOUS RHINOTRACHEITIS AGENT FROM OTHER STRAINS AND FIELD ISOLATES OF FELINE INFECTIOUS RHINOTRACHEITIS VIRUS BY ANALYSING MELTING TEMPERATURE CURVE OF PCR V1-GENE PRODUCTS USING ORANGE POLYMER DYE SUPERNOVA V605 | 2024 |
|
RU2824661C1 |
| METHOD FOR INDIRECT DETERMINATION OF TITER OF INFECTIOUS ACTIVITY OF CANINE MASTADENOVIRUS IN RAW MATERIALS FOR VACCINES USING REAL-TIME PCR | 2023 |
|
RU2808585C1 |
| METHOD FOR MEDIATED DETERMINATION OF THE INFECTIOUS TITER OF THE RABIES VIRUS IN NON-INACTIVATED RAW MATERIALS FOR RABIES VACCINES IN TRANSCRIPTIONAL AMPLIFICATION AND DETECTION OF REACTION PRODUCTS USING THE BEACON TECHNOLOGY | 2020 |
|
RU2756472C1 |
