METHOD FOR PREDICTING DISEASE SEVERITY AND OUTCOME IN PATIENTS WITH SURGICAL SEPSIS Russian patent published in 2018 - IPC G01N33/53 

FIELD: medicine.

SUBSTANCE: present invention relates to medicine, namely to diagnosing systemic inflammation and predicting severity and outcome of a disease in patients with surgical sepsis. For this, venous blood of patients are sampled in the first 48 hours after diagnosing sepsis from a peripheral vein into a test tube, K₃EDTA is added. No more than 2 hours after blood sampling, an expression level of C3aR is assessed quantitatively on monocytes by the average fluorescence intensity on a flow cytometer. No less than 10⁴ cells are analyzed in each sample, further in the course of treatment, blood is sampled again, expression level of C3aR is determined on monocytes and a prognostic index IC3aR_1–4 is calculated in the disease dynamics according to the formula: IC3aR₁ = N₂ / N₁, IC3aR₂ = N₃ / N₁, IC3aR₃ = N₄ / N₁, IC3aR₄ = N₅ / N₁, where IC3aR₁ – a prognostic index with accuracy of one decimal place in the disease dynamics, IC3aR_2–4 – a prognostic index in the disease dynamics, N₁ – an initial value of an expression index on C3aR monocytes (MFI) at the initial stage of the disease in the first 48 hours, N_2–5 – an expression value on C3aR monocytes (MFI) in subsequent studies in the disease dynamics. If IC3aR value is greater than 1.0 and IC3aR_2–4 increases with each subsequent study, dismal prognosis of disease severity and disease outcome in patients with surgical sepsis leading to death is made.

EFFECT: use of the claimed invention allows predicting the disease severity and outcome in patients with surgical sepsis.

1 cl, 3 ex