FIELD: medicine.
SUBSTANCE: invention relates to medicine. Method for determining markers for the presence of tumor T-lymphoblasts is proposed. PBMC fraction is isolated from the sample of the bone marrow of the patient with acute leukemia, genomic DNA extraction, multiplex polymerase chain reaction using isolated genomic DNA as a template and oligonucleotide primers for TRB loci, electrophoresis, determination of the number of V (D) J-rearrangements associated with tumor clones, the sequencing of eluted multiplex PCR products to determine the sequences of V (D) J-rearrangements of TRB, contained in the major products of multiplex PCR. Established sequences of V (D) J-rearrangements of TRB genes are identified markers of the presence of tumor T-lymphoblasts.
EFFECT: invention provides a determination of the type of predominant V (D) J-rearrangements of T cell receptor genes and their number in a diagnostic sample of individual genomic DNA.
1 cl, 1 dwg, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR TUMOUR B LYMPHOBLASTS AVAILABILITY MARKERS DETERMINATION | 2015 |
|
RU2638800C2 |
| METHOD OF DIAGNOSING ONCOLOGICAL DISEASES OF THE BLOOD | 2022 |
|
RU2803281C1 |
| METHOD FOR DETECTION OF STRUCTURAL REARRANGEMENTS OF TUMOR CELLS (CHEMERIC GENES) GENOME, DETERMINING SELECTION OF THERAPY AND PREDICTION IN ACUTE LEUKOSIS IN CHILDREN, USING OT-PCR AND SUBSEQUENT HYBRIDIZATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) | 2016 |
|
RU2639513C1 |
| METHOD OF CREATING TARGET PANEL FOR STUDYING GENOMIC REGIONS FOR DETECTING THERAPEUTIC BIOMARKERS OF IMMUNE CHECKPOINT (IC) INHIBITORS | 2023 |
|
RU2818360C1 |
| METHOD FOR ANALYSING DIFFERENTIALLY METHYLATED GENOMIC REGIONS IN BIOLOGICAL SAMPLES OF BONE MARROW AND BLOOD OF CHILDREN WITH ACUTE MYELOID LEUKAEMIA | 2017 |
|
RU2689727C2 |
| METHOD FOR MEASURING ARTIFICIAL IMMUNITY | 2010 |
|
RU2539032C2 |
| METHOD FOR ANALYSING SOMATIC MUTATIONS IN IDH1 AND IDH2 GENES USING LNA-BLOCKING PCR AND HYBRIDISATION WITH BIOLOGICAL MICROCHIP | 2024 |
|
RU2839291C1 |
| COMPOSITIONS AND METHODS OF MEASUREMENT AND CALIBRATION OF SYSTEMATIC MISTAKE OF AMPLIFICATION IN MULTIPLEX PCR-REACTIONS | 2013 |
|
RU2631797C2 |
| METHODS OF DETECTING AND MONITORING CANCER BY PERSONALIZED DETECTION OF CIRCULATING TUMOR DNA | 2019 |
|
RU2811503C2 |
| PREIMPLANTATION GENETIC TESTING METHOD FOR FANCONI ANEMIA | 2022 |
|
RU2792147C1 |
