FIELD: biotechnology.
SUBSTANCE: proposed plasmid pPICZαA-PhoA2-StV, determining the synthesis of phospholipase A2, having a size of 3,862 bp. and containing the phospholipase gene PhoA2-StV. Proposed strain of the yeast Pichia pastoris X-33, transformed with the indicated plasmid. Proposed the method of obtaining the enzyme preparation of phospholipase A2 using the specified strain.
EFFECT: group of inventions allows to expand the arsenal of means for obtaining phospholipase A2 and enzyme preparations based on it.
5 cl, 2 dwg, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| OBTAINING PHOSPHOLIPASE A2 GENE WITH CHANGED pH OPTIMUM BY REMOVING GLYCOSYLATION SITES | 2019 |
|
RU2766448C2 |
| METHOD FOR PRODUCING PHOSPHOLIPASE А2 KOMAGATAELLA PHAFFII (PICHIA PASTORIS) YIB ΔLEU2_PLA2S PRODUCTION STRAIN | 2020 |
|
RU2746817C1 |
| PHOSPHOLIPASE C ENZYME PREPARATION BASED ON RECOMBINANT PRODUCER STRAIN BACILLUS MOJAVENSIS RCAM05968 BDV-1 PLC 6H FOR USE IN FOOD INDUSTRY AND METHOD FOR PRODUCTION THEREOF | 2023 |
|
RU2822431C1 |
| METHOD FOR PURIFYING A RECOMBINANT ENZYME PREPARATION OF PHOSPHOLIPASE A2 FROM A PRODUCER STRAIN PICHIA PASTORIS | 2019 |
|
RU2746563C1 |
| PHOSPHOLIPASE A2 FOR EXPRESSION IN YEAST (EMBODIMENTS) | 2018 |
|
RU2716087C1 |
| METHOD FOR PRODUCING SECRETED FULLY FUNCTIONAL PHOSPHOLIPASE A2 IN SACCHAROMYCES CEREVISIAE YEAST, A PRECURSOR PROTEIN FOR IMPLEMENTING SAID METHOD (VERSIONS) | 2019 |
|
RU2728240C1 |
| RECOMBINANT PLASMID FOR EXPRESSION IN PICHIA PASTORIS YEAST GENE OF CHIMERIC PROTEIN OF HUMAN ANGIOGENIN AND STRAIN OF PICHIA PASTORIS YEAST - PRODUCER OF RECOMBINANT CHIMERIC PROTEIN OF HUMAN ANGIOGENIN | 2017 |
|
RU2658758C1 |
| RECOMBINANT PLASMID, METHOD FOR ITS CONSTRUCTION AND YEAST STRAIN KOMAGATAELLA PASTORIS - PRODUCER OF IMMUNE INTERFERON GAMMA OF A DOG | 2020 |
|
RU2756852C2 |
| RECOMBINANT PLASMID FOR PHOSPHOLIPASE GENE EXPRESSION IN PICHIA PASTORIS YEAST, PICHIA PASTORIS YEAST STRAIN-PHOSPHOLIPASE PRODUCER | 2009 |
|
RU2409671C1 |
| RECOMBINANT CHIMERIC PROTEIN SUMO3-apoA-I FOR PRODUCING MATURE HUMAN APOLIPOPROTEIN A-I, Pichia pastoris YEAST STRAIN - PRODUCER OF RECOMBINANT CHIMERIC PROTEIN SUMO3-apoA-I AND METHOD OF PRODUCING MATURE HUMAN APOLIPOPROTEIN A-I | 2015 |
|
RU2605326C1 |
