FIELD: biotechnology.
SUBSTANCE: disclosed is a genetically engineered microorganism capable of converting cholesterol, analogues and derivatives of cholesterol into steroid hormone precursors, where said microorganism comprises at least one DNA sequence which codes cytochrome P450 of eukaryotic origin, exogenous DNA sequence which codes Adx, and exogenous DNA sequence which codes AdR, and wherein said microorganism is Bacillus megaterium. Disclosed is a method of producing steroid hormone precursors using said microorganism. Disclosed is a method of producing recombinant strains of a Bacillus megaterium microorganism, which are improved with respect to the conversion of cholesterol, analogues and derivatives of cholesterol into steroid hormone precursors.
EFFECT: group of inventions increases output of steroid hormone precursors compared to other systems producing said product.
14 cl, 16 dwg, 1 tbl, 5 ex
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Year |
Author |
Number |
| NOVEL CYTOCHROME P450 POLYPEPTIDE WITH INCREASED ENZYMATIC ACTIVITY |
2015 |
|
RU2677997C2 |
| NUCLEIC ACID SEQUENCE ENCODING PROTEIN ELICITING DELTA-5,7-STEROL,DELTA-7 REDUCTASE ACTIVITY (VARIANTS), PROTEIN FROM ARABIDOPSIS THALIANA ELICITING INDICATED ACTIVITY (VARIANTS), EXPRESSION VECTOR, METHOD FOR CLONING NUCLEIC ACID, DIAGNOSTIC PROBE, METHOD FOR PREPARING PROTEIN, METHOD FOR REDUCING STEROL (VARIANTS), METHOD FOR PREPARING PREGNENOLONE (VARIANTS), TRANSFORMED YEAST STRAIN AND METHOD FOR DETECTING INSUFFICIENCY OF DELTA-5,7-STEROL,DELTA-7 REDUCTASE |
1996 |
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|
RU2242517C2 |
| EXPRESSION METHOD |
2013 |
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|
RU2644199C2 |
| RECOMBINANT PLASMID PGBP450F FOR PREPARING TRANSGENIC PLANTS AND METHOD FOR PREPARING TRANSGENIC TOBACCO PLANTS WITH ENHANCED PRODUCTIVITY AND RESISTANCE TO FUNGAL PHYTOPATHOGENS |
2002 |
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|
RU2237717C2 |
| CHOLESTEROL-PRODUCING YEAST STRAINS AND USE THEREOF |
2005 |
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|
RU2397245C2 |
| NOVEL CYTOCHROME P-450 MONOOXYGENASES AND THEIR USING FOR OXIDATION OF ORGANIC COMPOUNDS |
2000 |
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|
RU2285044C2 |
| FODDER ADDITIVE WITH PROBIOTIC ACTIVITY FOR FARM ANIMALS, BIRDS, HORSES AND FISH |
2017 |
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|
RU2652836C1 |
| MICROORGANISM FOR PRODUCING L-AMINO ACID, HAVING HIGH ACTIVITY OF CYTOCHROME C, AND METHOD OF PRODUCING L-AMINO ACID USING SAME MICROORGANISM |
2020 |
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RU2819270C1 |
| PRODUCING TERMINAL ALKENES VIA ENZYMATIC DECARBOXYLATION OF 3-HYDROXYALKANOIC ACIDS |
2009 |
|
RU2571933C2 |
| FUCOSYLTRANSFERASES AND THEIR USE FOR OBTAINING FUCOSYLATED OLIGOSACCHARIDES |
2018 |
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RU2818835C2 |
