NUCLEIC ACID SEQUENCE ENCODING PROTEIN ELICITING DELTA-5,7-STEROL,DELTA-7 REDUCTASE ACTIVITY (VARIANTS), PROTEIN FROM ARABIDOPSIS THALIANA ELICITING INDICATED ACTIVITY (VARIANTS), EXPRESSION VECTOR, METHOD FOR CLONING NUCLEIC ACID, DIAGNOSTIC PROBE, METHOD FOR PREPARING PROTEIN, METHOD FOR REDUCING STEROL (VARIANTS), METHOD FOR PREPARING PREGNENOLONE (VARIANTS), TRANSFORMED YEAST STRAIN AND METHOD FOR DETECTING INSUFFICIENCY OF DELTA-5,7-STEROL,DELTA-7 REDUCTASE Russian patent published in 2004 - IPC

FIELD: genetic engineering, biochemistry, molecular biology, medicine.

SUBSTANCE: method involves to cloning procedure of nucleotide sequence in yeast that provides preparing protein eliciting activity of delta-5,7-sterol,delta-7 reductase. Reduction of sterol at position C-7 can be used for preparing pregnenolone and hydrocortisone and using the corresponding hybridizing probe can be used for detection of insufficiency of delta-5,7-sterol,delta-7 reductase activity in human body, for example, in inherent failure of this enzyme, and in patients suffering with different diseases, for example, with clinical symptoms of syndrome RSH/SLO also.

EFFECT: valuable medicinal properties of nucleic acid sequence, improved cloning method and method for protein preparing.

11 cl, 27 dwg, 2 tbl, 4 ex