METHODS OF DETECTING AND QUANTIFYING NUCLEIC ACIDS WITHIN MALLEABLE POLYMERS Russian patent published in 2019 - IPC C12N15/10 C12Q1/68 

Abstract RU 2679353 C2

FIELD: biotechnology.

SUBSTANCE: proposed is a method of extracting nucleic acids from a polymer that is malleable within a living organism, method of detecting a microorganism adhered or entrapped to the polymer malleable within a living organism, method of identifying malleable polymers resistant to adherence of a microorganism, method of generating a microbe profile of the oral cavity of a mammalian subject and an in vitro method of diagnosing an infection in the oral cavity of a mammal and determining the susceptibility of a mammal for developing an infection in the oral cavity. Said methods include contacting the polymer with chloroform and a buffer solution, extracting chloroform from the buffer solution, where the extracted nucleic acids are in the buffer solution.

EFFECT: invention provides for the detection, identification and quantification of microorganisms adhered or entrapped to the polymer malleable within a living organism.

24 cl, 1 dwg, 3 tbl, 2 ex

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RU 2 679 353 C2

Authors

Majtra Amarnat

Morando Devid

Dates

2019-02-07Published

2014-03-13Filed