FIELD: medicine.
SUBSTANCE: group of inventions relates to medicine, namely to oncology, and can be used to diagnose the detection of Ph-negative myeloproliferative blastoma. A set containing specific primers and fluorescence-labelled DNA probes is proposed. Primers have the following nucleotide composition (5'-3'-): JAK2-W-F, JAK2-W-R, JAK2-W-P, JAK2-M-F, JAK2-M-R, JAK2-M-P, CALR-l-F, CALR-l-R, MPL-K-F, MPL-K-R, CALR-1-P, MPL-K-P, CALR-2-F, CALR-2-R, MPL-L-F, MPL-L-R, CALR-2-P, MPL-L-P. In TaqMan probes on 5' end dyes are used: fluorescein (FAM) and chlorinated fluorescein (HEX). For extinction of fluorescence, Black Hole Quencher-1 (BHQ1) on 3' end is used. Also a diagnostic method of Ph-negative myeloproliferative blastoma is proposed, including a conduction of a real-time polymerase chain reaction.
EFFECT: group of inventions allows the simultaneous study of several mutations in one analytical series, which leads to the result in a shorter period of time; and also provides an accurate diagnosis of chronic Ph-negative myeloproliferative blastoma, allows to identify a group of patients with the presence of combined pathogenetic mutations.
2 cl, 3 dwg, 3 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SET OF REAGENTS FOR SIMULTANEOUS DETECTION OF FIVE MAJOR SOMATIC MUTATIONS IN pH-NEGATIVE MYELOPROLIFERATIVE NEOPLASMS AND DIAGNOSTIC METHOD BASED ON IT | 2022 |
|
RU2802421C1 |
| METHOD FOR PREPARING SET OF STANDARD SAMPLES FOR QUALITY CONTROL OF MOLECULAR GENETIC DIAGNOSIS OF pH-NEGATIVE MYELOPROLIFERATIVE DISEASES | 2024 |
|
RU2832111C1 |
| METHOD FOR DETERMINING MUTATIONS IN THE CALR GENE BY ANALYZING MELTING CURVES IN THE PATIENTS SUFFERING CHRONIC MYELOPROLIFERATIVE DISEASES | 2019 |
|
RU2722077C1 |
| REAGENT SET FOR ESTIMATING EFFICIENCY OF THERAPY WITH TYROSINE KINASE INHIBITORS AT Ph-POSITIVE NEOPLASMS AND ITS APPLICATION METHOD | 2020 |
|
RU2762356C2 |
| TEST SYSTEM FOR IDENTIFYING A MUTATION IN THE BTPKD GENE OF THE DOMESTIC DOG (CANIS LUPUS FAMILIARIS), CAUSING POLYCYSTIC KIDNEY DISEASE | 2020 |
|
RU2763933C1 |
| OLIGONUCLEOTIDE SEQUENCE OLIGONUCLEOTIDE SEQUENCE FOR THE NRAS GENE Q61R MUTATION IN THE TUMOR FORMATIONS OF THE THYROID GLAND | 2019 |
|
RU2688189C1 |
| MUTANT CALRETICULIN FOR DIAGNOSIS OF MYELOID MALIGNANCIES | 2014 |
|
RU2668808C2 |
| SET OF REAGENTS FOR THE DETERMINATION OF RIBONUCLEIC ACIDS IN THE DIAGNOSIS OF LYMPHOPROLIFERATIVE DISEASES | 2023 |
|
RU2802064C1 |
| METHOD FOR ANALYSIS OF SOMATIC MUTATIONS IN GNAQ AND GNA11 GENES USING LNA-BLOCKING MULTIPLEX PCR AND SUBSEQUENT HYBRIDIZATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) | 2017 |
|
RU2674687C1 |
| METHOD OF ANALYZING TERMINAL MUTATIONS IN BRCA1, BRCA2, ATM AND PALB2 GENES USING MULTIPLEX PCR AND SUBSEQUENT HYBRIDISATION WITH AN OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) | 2020 |
|
RU2729360C1 |
