FIELD: biotechnology.
SUBSTANCE: described is a test system for identifying a mutation (SNP: 6:38856816; NC_006588.3:g.38856816G>A) in the BTPKD gene, causing polycystic kidney disease in dogs. The test system includes a mixture for conducting a polymerase chain reaction using the developed primers, Taqman probes of the diagnosed allele, and "plug" oligonucleotides for another allele, presented below: Primer F - CCTCAAGCCTGTCCATCTGT; Primer R - GGAGAGCCAGATGTGCTTGT; Probe mut - FAM-TCCTCGTGGCCAAGCTGCAG-BHQ1; Plug wt - CCTCGTGGCCGAGCTGCAG-NH2; Probe wt - FAM - CCTCGTGGCCGAGCTGCAG-BHQ1; Plug mut - TCCTCGTGGCCAAGCTGCAG-NH2.
EFFECT: invention expands the range of means of identifying mutations causing polycystic kidney disease in dogs.
1 cl, 2 dwg, 1 tbl, 1 ex
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