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2 681 229

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IPC

C07K19/00(2006-01-01)

C07K14/20(2006-01-01)

C12N15/31(2006-01-01)

C12N15/63(2006-01-01)

G01N33/569(2006-01-01)

(21) (22)

Application

2017138441, 2017-11-03

(24)

Start date

2017-11-03

(22)

Actual filing date

2017-11-03

(45)

Published

2019-03-05

(72)

Inventor

Panfertsev Evgenij AleksandrovichBaranova Evgeniya VladimirovnaMochalov Vladimir VyacheslavovichBiketov Sergej FedorovichSolovev Pavel VladimirovichGorbatov Aleksej Aleksandrovich

(73)

Holder

Federalnoe Byudzhetnoe Uchrezhdenie Nauki Gosudarstvennyj Nauchnyj Tsentr Prikladnoj Mikrobiologii I Biotekhnologii Gnts Pmb)

NUCLEOTIDE SEQUENCE ENCODING THE DBPAG POLYPEPTIDE, ADVANTAGEOUS FOR SERODIAGNOSIS OF LYME BORRELIOSIS, PETDBPAGNH RECOMBINANT PLASMID DNA, ENCODING DBPAG COMBINED POLYPEPTIDE, RECOMBINANT BACTERIA STRAIN ESCHERICHIA COLI BL21(DE3)/PETDBPAGNH - PRODUCER OF DBPAG POLYPEPTIDE, DBPAG POLYPEPTIDE AND METHOD FOR PREPARATION THEREOF Russian patent published in 2019 - IPC C07K19/00 C07K14/20 C12N15/31 C12N15/63 G01N33/569

Abstract RU 2681229 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology, specifically to the production of an immunogenic polypeptide that provides for the formation of an immune response against infection caused by spirochetes of the Borrelia burgdorferi sensu lato complex, and can be used for serodiagnosis of tick-borne borreliosis. Recombinantly, in cells of the E. coli BL21 (DE3)/pETdbpagNH strain of bacteria, a DBPAG fusion polypeptide consisting of the immunogenic domains of the Borrelia garinii and Borrelia afzelii DBPA proteins is obtained.

EFFECT: invention provides a new polypeptide that is advantageous for the development of a new test system for the diagnosis of Lyme borreliosis.

5 cl, 5 dwg, 2 tbl, 5 ex

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RU 2 681 229 C1

Authors

Panfertsev Evgenij Aleksandrovich

Baranova Evgeniya Vladimirovna

Mochalov Vladimir Vyacheslavovich

Biketov Sergej Fedorovich

Solovev Pavel Vladimirovich

Gorbatov Aleksej Aleksandrovich

Dates

2019-03-05—Published

2017-11-03—Filed