TARGET SEQUENCE DETECTION BY NANOPORE SENSING OF SYNTHETIC PROBES Russian patent published in 2019 - IPC C12Q1/68 G01N33/487 G01N27/00 B82Y5/00 

FIELD: biotechnology.

SUBSTANCE: method for detecting a polynucleotide or polynucleotide sequence in a sample has been proposed. Said method includes bringing a sample into contact with the first and second probe, where the first and second probe specifically bind to the first and second target polynucleotide sequence, respectively, bringing the sample into contact with the third molecule to form the containing polynucleotide, the first and second probe, the third molecule of the fusion complex, loading the sample into the first chamber of the nanopore device and determining the presence or absence of a complex in the sample. Moreover, the nanopore device contains a nanopore and the first chamber and the second chamber that are in electrical and liquid communication by means of a nanopore. In addition, the nanopore device is characterized by controlled voltage between the nanopore and the sensor associated with the nanopore, where the sensor is made with the possibility of identifying objects passing through the nanopore.

EFFECT: invention provides specific binding of the probe to the sequence for its detection in the nanopore.

21 cl, 41 dwg, 1 tbl, 7 ex