FIELD: biotechnology.
SUBSTANCE: group of inventions relates to genetics and can be used for polynucleotide sequencing. That is ensured by using a polynucleotide sequencing composition comprising: a nanopore; plurality of nucleotides; first and second polynucleotides; polymerase; long-term connecting structure; a first fragment located on the elongated body; reporter region. Polynucleotide sequencing system comprising the composition and the polynucleotide sequencing method itself are also used.
EFFECT: this group of inventions is more reliable, reproducible and sensitive.
43 cl, 1 tbl, 12 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| SUPPRESSING ERRORS IN SEQUENCED DNA FRAGMENTS BY USING EXCESSIVE READING WITH UNIQUE MOLECULAR INDICES (UMI) | 2016 |
|
RU2704286C2 |
| NUCLEASE-MEDIATED DNA ASSEMBLY | 2015 |
|
RU2707911C2 |
| METHOD AND COMPOSITION FOR ANALYZING CELLULAR COMPONENTS | 2016 |
|
RU2717491C2 |
| METHOD AND COMPOSITION FOR ANALYSIS OF CELLULAR COMPONENTS | 2016 |
|
RU2761432C2 |
| COMPLEX SYSTEMS OF TRANSPOSOME BOUND ON SURFACE | 2020 |
|
RU2790295C2 |
| METHODS AND DEVICES FOR DETECTION AND IDENTIFICATION OF ENCODED GRANULES AND BIOLOGICAL MOLECULES | 2007 |
|
RU2487169C2 |
| METHODS AND COMPOSITIONS FOR DETERMINING LIGANDS ON MATRICES USING INDICES AND BARCODES | 2019 |
|
RU2816708C2 |
| DETECTION OF NUCLEOTIDE VARIATIONS IN NUCLEIC ACID SEQUENCE-TARGETS IN THE ANALYSIS WITH THE CLEAVAGE AND EXTENSION OF THE PROBING AND TAGGING OLIGONUCLEOTIDE (PTO) | 2013 |
|
RU2620955C2 |
| DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY PTO CLEAVAGE AND EXTENSION ASSAY | 2012 |
|
RU2566562C2 |
| LIBRARIES FOR NEXT GENERATION SEQUENCING | 2014 |
|
RU2698125C2 |
