NOVEL COMPOUNDS AND WAYS OF USING THEM FOR DETECTING TARGET MOLECULES IN A SAMPLE Russian patent published in 2022 - IPC C07D401/12 C08G63/48 C12Q1/00 

FIELD: chemistry.

SUBSTANCE: invention relates to a profluorophore of formula (I), where R₁ is H; R₂-R₈, R₁₁-R₁₂ and R₁₄ are independently selected from hydrogen, hydroxyl, halogen, cyano, nitro, optionally substituted C₁-C₁₀alkyl, optionally substituted amino-C₁-C₁₀alkyl, optionally substituted C₁-C₁₀alkoxy, R₁₃ is selected from optionally substituted C₁-C₁₀alkyl, such as optionally substituted ethyl, optionally substituted propyl or optionally substituted butyl; R₁₅ is H; Z is -CR₁₆R₁₇, where R₁₆ and R₁₇ are independently selected from hydrogen and optionally substituted C₁-C₆alkyl, or any tautomer, stereoisomer, salt thereof, where the term "substituted" refers to groups substituted with 1–5 substituents selected from the group, consisting of "C₁-C₆alkyl", "amino", "aminosulphonyl", "aminocarbonyl", "sulphonyl", "alkoxy", "alkoxycarbonyl", "halogen", trihalogenomethyl, cyano, hydroxy, mercapto and nitro. Invention also relates to conjugates based on a profluorophore of formula (I) and use thereof to detect a target molecule in a sample, particularly target nucleic acid molecules. Invention also relates to novel fluorophores of formula (I)' and a method of producing them.

EFFECT: obtaining novel profluorophores, which provide high-efficiency detection and are suitable for portable and disposable detection systems, which can be used in diagnostics and quality control.

25 cl, 9 dwg, 10 ex