METHOD OF PRODUCING CONJUGATE BASED ON MAGNETIC METALL-CARBON NANOPARTICLES SUITABLE FOR DIAGNOSTIC AND ANALYTICAL TARGET USING NMR-RELAXOMETRY AS A DETECTION METHOD Russian patent published in 2019 - IPC G01N33/553 B01D15/34 B82B3/00 

FIELD: biotechnology.

SUBSTANCE: invention relates to molecular immunobiotechnology and is intended for functionalizing the surface of magnetic metal-carbon nanoparticles. Sorption is carried out in conditions of intensive mechanical mixing of functional groups of water-soluble polymer on the surface of magnetic nanoparticles. Polymer molecules are covalently cross-linked to form a strong chemically structured "frame" that is accompanied by simultaneous chemical activation of peptized particles. Peptized activated particles are mixed with a solution containing streptavidin molecules and a biotinylated probe specific to the compound to be determined, or G protein of streptococci and antibodies specific to the determined compound for conjugation, after which the ready conjugate is released from unreacted molecules of the mixture by gel-permeation chromatography.

EFFECT: invention provides higher sensitivity and information content of the method and can serve as a basis for creating highly sensitive diagnostic testing systems using the NMR-relaxometry method.

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