FIELD: chemistry.
SUBSTANCE: according to the method magnetic nanoparticles and glutaric dialdehyde are added to a polymer in a phosphate buffer under conditions of an alternating magnetic field, then, peptised and activated particles are three times washed with a phosphate buffer solution, depositing them under the influence of a constant magnet, the activated particles are mixed with an anti-analite solution for conjugation under conditions of the alternating magnetic field, after which the conjugate is washed three times by deposition-resuspension in the phosphate buffer solution, alternating the action of constant and alternating magnetic fields.
EFFECT: obtaining strong functional conjugates which can serve as a basis for the creation of effective instrument-free systems for simplified diagnostic testing.
4 ex
Title | Year | Author | Number |
---|---|---|---|
METHOD OF PRODUCING CONJUGATE BASED ON MAGNETIC METALL-CARBON NANOPARTICLES SUITABLE FOR DIAGNOSTIC AND ANALYTICAL TARGET USING NMR-RELAXOMETRY AS A DETECTION METHOD | 2018 |
|
RU2684325C1 |
METHOD FOR PRODUCING CONJUGATE FOR PERFORMING STEREOSPECIFIC ANALYSIS | 2005 |
|
RU2314827C2 |
METHOD OF STEREOSPECIFIC ANALYSIS AND A METHOD OF PREPARING CONJUGATE FOR STEREOSPECIFIC ANALYSIS | 1993 |
|
RU2089912C1 |
METHOD FOR DETECTING IMMUNOREACTIVE COMPOUNDS | 2004 |
|
RU2268471C2 |
INSTRUMENT-FREE DIAGNOSTIC TECHNIQUE FOR PSEUDOTUBERCULOSIS | 2011 |
|
RU2464573C1 |
COMPOSITE PLAGUE ANTIGEN F1-DIAGNOSTICUM FOR INDICATION OF SPECIFIC ANTIBODIES | 2015 |
|
RU2582941C1 |
METHOD OF SYNTHESIS OF FUNCTIONAL CARBON QUANTUM DOTS | 2023 |
|
RU2824005C1 |
METHOD FOR ESTIMATING LEVEL OF POST-VACCINE IMMUNITY TO INFLUENZA VIRUS | 2013 |
|
RU2533272C1 |
CONJUGATE OF LUMINESCENT SILICON DIOXIDE NANOPARTICLES WITH RECOMBINANT SINGLE-DOMAIN C7B LAMA NANO-MONOANTIBODIES, CAPABLE OF SPECIFICALLY INTERACTING WITH RECOMBINANT HER2/NEU PROTEIN - ANALOGUE OF EPIDERMAL GROWTH FACTOR NATURAL RECEPTOR EGFR/ERBB OF HUMAN CELLS | 2017 |
|
RU2679075C1 |
METHOD OF PREPARING SOLUBLE CONJUGATES OF BIOLOGICALLY ACTIVE SUBSTANCES | 2000 |
|
RU2196605C2 |
Authors
Dates
2015-03-10—Published
2013-07-23—Filed