METHOD OF REMOVING ENDOTOXINS FROM BIOLOGICAL FLUIDS USING COVALENTLY IMMOBILIZED LYSOZYME AS LIGAND Russian patent published in 2019 - IPC C12N9/36 C12N11/10 G01N33/573 

FIELD: chemistry.

SUBSTANCE: invention relates to techniques for using sorbents used for medical purposes, particularly for extracorporeal therapy in patients with sepsis using biological fluid sorption. Objective is solved by the proposed method of endotoxin removal (sorption) from biological fluids by means of chemically (covalently) immobilized in the aminated agarose matrix of lysozyme as a ligand. Immobilisation of lysozyme on an agarose aminated matrix is carried out to obtain a molecular spacer -NH-C₆H₁₂-NH-C₅H₁₀-NH-, which connects a lysozyme molecule.

EFFECT: practical implementation of the idea of using immobilized lysozyme as a ligand in a hemocompatible sorbent matrix for removing endotoxins from biological fluids with no risk of leucocyte leaching into the sorbed biological fluid (aqueous solution, including saline solution, blood plasma and whole blood (taking into account sorbent hemocompatibility)).

1 cl, 1 dwg, 9 tbl