METHOD FOR DETERMINING CEFOTAXIME BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY Russian patent published in 2019 - IPC G01N30/02 G01N30/10 G01N30/26 G01N30/50 G01N30/74 C07D501/06 C07D501/24 C07D417/12 

FIELD: technological processes.

SUBSTANCE: present invention relates to a method of determining cefotaxime by reversed-phase high-performance liquid chromatography, comprising an isocratic mode of elution using a chromatographic column filled with a sorbent with particle size of 5 mcm, used as a mobile phase is a mixture of a solution of ammonium acetate with acetonitrile, characterized by the fact that chromatographic separation is carried out on a column with size of 250×3 mm, filled with sorbent C18, using as a mobile phase mixture of 0.02 M ammonium acetate solution pH = 4.7 with acetonitrile in ratio of 90:10 using ultraviolet detector at wave length 252 nm and volume of introduced sample 10 mcl.

EFFECT: effective separation of cefotaxime with impurity compounds of the sample and providing high retention of cefotaxime, which increases sensitivity and resolution of the method, reduced consumption of the mobile phase, which increases cost-effectiveness of analysis.

1 cl, 2 dwg, 1 ex