METHOD FOR DIAGNOSIS OF MICROCIRCULATORY AND TISSUE DISORDERS IN FEET OF PATIENTS WITH DIABETES MELLITUS Russian patent published in 2019 - IPC A61B5/1455 A61B8/06 

FIELD: medicine.

SUBSTANCE: invention refers to functional diagnostics, and can be used for diagnosing microcirculatory-tissue disorders in the footsteps of patients with diabetes mellitus. Biological tissue is exposed on the dorsal surface of the patient's foot at a point located on plateau between 1st and 2nd metatarsal bones. Exposure is performed once at wavelength of 365 nm for fluorescence excitation and once at wavelength of 1064 nm to determine dynamic blood microcirculation parameters. To assess microcirculation-tissue disorders at the first stage of analysis by fluorescence spectroscopy, fluorescence intensity of the analyzed biotissue region, which is excited at wavelength of 365 nm with a maximum fluorescence amplitude at wave length 460 ± 10 nm. Calculating the normalized amplitudes I₄₆₀. At the second stage, a heat sample is carried out using a temperature exposure channel. First, foot biotissue is cooled to 25 °C for 4 minutes to bring skin temperature in the examination area in all subjects to the same initial conditions. Then heated to 42 °C for 10 minutes. At the stage of the heat test, the blood microcirculation index is recorded by laser Doppler flowmetry. Average blood microcirculation I_m^42 °C is calculated. Using classification model in form of two discriminant functions D₁ and D₂ in accordance with formula

At position of point on plane to the right of line D₁ conclusion is made on the absence of microcirculatory-tissue disorders. If the point on the plane is positioned between the lines D1 and D2, microcirculatory tissue disorders are stated without the current risk of trophic ulcers. If the point on the plane is positioned above the D2 line, the presence of serious microcirculatory-tissue disorders in the feet with the ulcer and ulcer changes is stated.

EFFECT: method provides an integrated approach to assessing microcirculatory-tissue disorders in diabetic patients, assessing the general state of microcirculatory-tissue systems at earlier stages of the disease.

1 cl, 2 dwg, 1 tbl, 2 ex