METHOD OF PRODUCING A SUBSTANCE EXHIBITING ANTIFUNGAL ACTIVITY Russian patent published in 2019 - IPC A61K36/49 B01D11/02 A61P31/10 

FIELD: pharmaceuticals.

SUBSTANCE: invention refers to pharmaceutical industry, namely to a method for preparing a substance exhibiting antifungal activity. Method of producing a substance having antifungal activity, which is 7-O-methyl kaempferol, by preparing herbal raw material – horse-chestnut sprout (Aesculus hippocastanum L.), extraction of raw materials with organic solvents, evaporation and chromatographic purification, wherein extraction from air-dry vegetable raw material is carried out using chloroform in Soxhlet apparatus in ratio of raw material:extractant 1:3, subsequent extraction of the end substance from thickened chloroform extraction is carried out by column chromatography on silica gel using a mixture of chloroform and ethyl alcohol as eluent in ratio of 99:1 with volume of 1.5 l, and further purification of the target substance is carried out by recrystallisation of evaporated under vacuum eluates containing 7-O-methyl kaempferol, a mixture of chloroform and ethyl alcohol.

EFFECT: method described above enables to obtain a high yield of 7-O-methyl kaempferol – 1½ % of the weight of the initial air-dry material.

1 cl, 1 dwg