DETERMINATION OF NUCLEIC ACIDS BY AMPLIFICATION BASED ON INCORPORATION INTO CHAIN Russian patent published in 2019 - IPC C12Q1/68 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology and molecular biology. Disclosed is a method of determining a target nucleotide sequence in a sample in the presence of at least one recombinase or an accessory protein or a recombinase cofactor capable of binding to a single-stranded DNA, comprising bringing said sample into contact with at least one oligonucleotide probe containing a fluorophore, a damper and a region complementary to said target nucleotide sequence, wherein the sequence of said oligonucleotide probe comprises at least 20 % of RNA nucleotides, modified nucleotides of RNA and / or nucleotides of PNA (peptide nucleic acid), wherein the length of the oligonucleotide probe is at least 8 nucleotides and less than 30 oligonucleotides, as well as a set and a method for diagnosing diseases.

EFFECT: invention can be used in medicine, since it has higher specificity of probe binding to single-stranded DNA matrix when diagnosing diseases.

13 cl, 8 dwg, 8 ex