FIELD: biotechnology.
SUBSTANCE: invention relates to a method for editing the genome of infectious bovine lumpy skin disease virus. Said method is carried out using Overlap-PCR and CRISPR-Cas9 technology, wherein the method involves: design of a primer sequence for the synthesis of a plasmid coding an RNA guide: cloning the sequences into the P1 plasmid; shuttle vector construction; transformation of MDBK cell culture with plasmid P1, coding RNA guide, shuttle vector and p-Cas9/No NLS; infection of MDBK cell culture with LSD virus; obtaining genetically modified infectious bovine lumpy skin disease virus.
EFFECT: invention provides editing the genome of infectious bovine lumpy skin disease virus in vivo with high accuracy.
2 cl, 12 dwg, 8 tbl, 5 ex
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