FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology. A transformant of the yeast Komagataella phaffii producing phytase of Cronobacter turicensis is proposed. The transformant contains the DNA sequence of the m-phyCt gene encoding the phytase of Cronobacter turicensis, or one of the variants of such a sequence due to the degeneracy of the genetic code.
EFFECT: resulting phytase retains at least 80% and about 100% activity when exposed to pepsin or trypsin, respectively, for 1 hour at 37°C and a protease/enzyme ratio of 0.1 by weight.
1 cl, 3 dwg, 1 tbl, 3 ex
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