FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. Disclosed is a method of producing bioethanol from cellulose-containing material. Method involves preliminary treatment of the raw material with diluted acid solution with concentration of 1–12 % at atmospheric pressure, combined stage of fermentative hydrolysis and alcohol fermentation, extraction of bioethanol from wort. At the stage of enzymatic hydrolysis with initial concentration of dry substances of 6–9 %, total concentration of dry substances is 15–27 % by portion introduction of substrate and enzyme preparations with interval of 4–8 hours. Stage is carried out until conversion of substrate into reducing substances by 40–50 %, after which ethanol of synthesizing microorganisms, ethanol biosynthesis stimulators are added and alcohol fermentation combined with enzymatic hydrolysis is performed.
EFFECT: invention increases output of the product and minimizes costs during the stages of distillation and rectification of bioethanol.
1 cl, 5 ex
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Authors
Dates
2019-09-30—Published
2018-09-27—Filed