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2 819 835

(13)

(51)

IPC

G01N33/50(2006-01-01)

C12Q1/68(2006-01-01)

C12Q1/6827(2018-01-01)

C12Q1/686(2018-01-01)

(21) (22)

Application

2023134977, 2023-12-25

(24)

Start date

2023-12-25

(22)

Actual filing date

2023-12-25

(45)

Published

2024-05-27

(72)

Inventor

Bushueva Olga YurevnaKobzeva Kseniya AndreevnaShilenok Irina VladimirovnaMedvedeva Marina Viktorovna

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Obrazovatelnoe Uchrezhdenie Vysshego Obrazovaniya Gosudarstvennyj Meditsinskij Universitet" Ministerstva Zdravookhraneniya Rossijskoj Federatsii

METHOD FOR GENOTYPING POLYMORPHIC LOCUS rs7951676 (GT) OF C11orf58 GENE IN PERSON BY REAL-TIME PCR USING ALLELE-SPECIFIC FLUORESCENT PROBES Russian patent published in 2024 - IPC G01N33/50 C12Q1/68 C12Q1/6827 C12Q1/686

Abstract RU 2819835 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, molecular genetic diagnostics, in particular, to a method for genotyping a polymorphic locus rs7951676 (G>T) of the C11orf58 gene in a human by real-time PCR using allele-specific fluorescent probes, providing for PCR using specially selected primers (forward 5'-TGTCAGGCTAAATCTCATTTGCT-3' and reverse 5'-CATTTTCTTTCCTAAAGGCCAGT-3') and probes with fluorophores (G-allele-specific fluorescent-labelled probe 5'-(FAM)CTCTCCATGAGATATACTTGA(RTQ1)-3' and T-allele-specific fluorescent-labelled probe 5'-(ROX)CTCTCCATGATATATACTTGA(BHQ2)-3') in an amplifier with fluorescence detection.

EFFECT: invention widens the range of methods for genotyping polymorphic variants of the C11orf58 gene, is characterized by simplicity, accuracy and low cost.

1 cl, 1 dwg

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RU 2 819 835 C1

Authors

Bushueva Olga Yurevna

Kobzeva Kseniya Andreevna

Shilenok Irina Vladimirovna

Medvedeva Marina Viktorovna

Dates

2024-05-27—Published

2023-12-25—Filed