FIELD: agriculture.
SUBSTANCE: invention relates to the agriculture. For microclonal propagation of potatoes, propagation of tubed potato plants is performed in vitro using a hormone-free nutrient medium with pH 5.7–5.8, containing micro salts and macro salts according to Murashige and Skoog, Fe2SO4, CaCl2, meso-inositol, thiamine, pyridoxine, nicotinic acid, saccharose, glucose, agar, biologically active substances of mycelial fungus Trichoderma lixii, with the following ratio of components: Macrosalt – 50 mg/l, Microsalt – 1 mg/l, Fe2SO4⋅7H2O – 5.0 mg/l, CaCl2⋅2H2O – 440 mg/l, meso-inositol – 100 mg/l, thiamine – 1.0 mg/l, pyridoxine – 1.0 mg/l, nicotinic acid – 0.5 mg/l, acarose – 25 g/l, glucose – 25 g/l, agar – 6 g/l, biologically active substances of mycelial fungus Trichoderma lixii – 0.5 mg/l.
EFFECT: invention makes it possible to increase efficiency of reproduction of healthy pottyral potato plants.
1 cl, 2 tbl
Authors
Dates
2019-10-11—Published
2018-03-12—Filed