FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and can be used to produce human glutamate oxalacetate transaminase. Disclosed is a recombinant strain of bacteria Escherichia coli rhGOT1-His VKPM No. B-12963, which produces human glutamate oxaloacetate transaminase with the amino acid sequence SEQ ID NO:2, obtained by transformation of strain BL21 (DE3) Rosetta2pLysS with plasmid pET22b containing a cloned sequence GOT1 with ten C-terminal histidines.
EFFECT: invention provides a high level of expression of the target protein in a soluble active form characterized by a high level of enzyme activity.
1 cl, 3 dwg, 1 ex
| Title |
Year |
Author |
Number |
| pET-mChBac75Na PLASMID VECTOR, ESCHRICHIA COLI BL21(DE3/ pET-mChBac75Na BACTERIA STRAINS FOR CHBAC7NA MINIBACTENECIN ANTIMICROBIAL PEPTIDE EXPRESSION AND METHODS FOR PRODUCTION OF THESE PEPTIDES |
2016 |
- Balandin Sergej Vladimirovich
- Bolosov Ilya Aleksandrovich
- Panteleev Pavel Valerevich
- Kokryakov Vladimir Nikolaevich
- Shamova Olga Valerevna
- Ovchinnikova Tatyana Vladimirovna
|
RU2618850C2 |
| PEPTINOGLYCANE HYDROLASE, EXPRESSION PLASMID, CONTAINING PEPTIDOGLYCANE HYDROLASE-CODING DNA FRAGMENT, BACTERIUM-PRODUCER AND METHOD OF MICROBIOLOGICAL SYNTHESIS OF PEPTIDOGLYCANE HYDROLASE |
2012 |
- Legotskij Sergej Aleksandrovich
- Miroshnikov Konstantin Anatol'Evich
- Kabanov Aleksandr Viktorovich
- Kljachko Natal'Ja L'Vovna
|
RU2547584C2 |
| DNA CODING FULL-SIZE HUMAN INTERLEUKIN-RECEPTOR ANTAGONIST 36, DNA CODING FULL-SIZE HUMAN INTERLEUKIN-RECEPTOR ANTAGONIST 36 WITH C-END POLIGISTIDINE TAG, PLASMID EXPRESSION VECTOR (VERSIONS), ESCHERICHIA COLI BL21 [DE3] STAR (PET-IL36RAF),-PRODUCER OF FULL-SIZE RECEPTOR ANTAGONIST 36 AND STRAIN OF BACTERIA ESCHERICHIA COLI BL21 [DE3] STAR (PET-IL36RAF-HIS),-PRODUCER OF FULL-SIZE RECEPTOR ANTAGONIST 36 WITH C-END POLIGISTIDINE TAG |
2014 |
- Kondrateva Ekaterina Vladimirovna
- Nimiritskij Petr Petrovich
- Petrov Aleksandr Vladimirovich
- Simbirtsev Andrej Semenovich
|
RU2582569C2 |
| ESCHERICHIA COLI BL21(DE3)PLYSS/PET15B-HISCPF1 STRAIN: PRODUCER OF RNA-GUIDED ENDONUCLEASE CRISPR/CPF1 |
2021 |
- Vasikhovskaya Valeriya Aleksandrovna
- Romanenko Margarita Vladimirovna
- Netesov Sergej Viktorovich
|
RU2774120C1 |
| RECOMBINANT PLASMID DNA pEstPc, ENCODING POLYPEPTIDE WITH PROPERTIES OF ESTERASE Psychrobacter cryohalolentis K5, Escherichia coli BACTERIA STRAIN - PRODUCER OF POLYPEPTIDE WITH PROPERTIES OF ESTERASE Psychrobacter cryohalolentis K5
|
2011 |
- Novototskaja-Vlasova Ksenija Aleksandrovna
- Petrovskaja Lada Evgen'Evna
- Shcherbakova Viktorija Arturovna
- Rivkina Elizaveta Mikhajlovna
- Gilichinskij David Abramovich
|
RU2478708C1 |
| STRAIN OF ESCHERICHIA COLI BACTERIA - PRODUCER OF IL-29 RECOMBINANT PROTEIN |
2020 |
- Vasin Andrej Vladimirovich
- Gyulikhandanova Nataliya Evgenevna
- Lozhkov Aleksej Aleksandrovich
- Taraskin Aleksandr Sergeevich
- Elshin Nikita Dmitrievich
- Zabrodskaya Yana Aleksandrovna
- Klotchenko Sergej Anatolevich
- Plotnikova Marina Aleksandrovna
- Shaldzhyan Aram Arutyunovich
|
RU2760585C1 |
| RECOMBINANT PLASMID DNA PET19B-SAV, PROVIDING SYNTHESIS OF FULL-LENGTH STREPTAVIDIN STREPTOMYCES AVIDINII PROTEIN, BACTERIAL STRAIN ESCHERICHIA COLI - PRODUCER OF SOLUBLE FULL-LENGTH PROTEIN OF STREPTAVIDIN STREPTOMYCES AVIDINII |
2019 |
- Bashmakova Evgeniya Evgenevna
- Frank Lyudmila Alekseevna
- Kudryavtsev Aleksandr Nikolaevich
|
RU2728652C1 |
| RECOMBINANT STRAIN OF ESCHERICHIA COLI - PRODUCER OF ENDOGLUCANASES FROM THIELAVIA TERRESTRIS FUNGUS AND METHOD OF MICROBIOLOGICAL SYNTHESIS OF ENDOGLUCANASES BASED ON THIS STRAIN |
2022 |
- Selimzyanova Alina Ildarovna
- Rykov Sergej Viktorovich
- Berezina Oksana Valentinovna
|
RU2811434C1 |
| RECOMBINANT ESCHERICHIA COLI STRAIN - PRODUCER OF XYLOGLUCANASE FROM ASPERGILLUS CERVINUS FUNGI AND METHOD FOR XYLOGLUCANASE MICROBIOLOGICAL SYNTHESIS BASED ON THIS STRAIN |
2016 |
- Rykov Sergej Viktorovich
- Berezina Oksana Valentinovna
- Sakhibgaraeva Liliya Flyuzovna
- Krestyanova Irina Nikolaevna
- Yarotskij Sergej Viktorovich
|
RU2625013C1 |
| RECOMBINANT PLASMID DNA pEst877 DETERMINING EXPRESSION OF POLYPEPTIDE WITH ACTIVITY OF ESTERASE Psychrobacter cryohalolentis K5 ON CELL SURFACE Escherichia coli, AND BACTERIA STRAIN Escherichia coli BL21 (DE3) pLysS/pEst877-PRODUCER OF POLYPEPTIDE WITH ACTIVITY OF ESTERASE PSYCHROBACTER CRYOHALOLENTIS K5 ON CELL SURFACE |
2013 |
- Petrovskaja Lada Evgen'Evna
- Krjukova Elena Aleksandrovna
- Novototskaja-Vlasova Ksenija Aleksandrovna
- Rivkina Elizaveta Mikhajlovna
- Dolgikh Dmitrij Aleksandrovich
- Kirpichnikov Mikhail Petrovich
|
RU2526213C1 |
