FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and can be used to produce human glutamate oxalacetate transaminase. Disclosed is a recombinant strain of bacteria Escherichia coli rhGOT1-His VKPM No. B-12963, which produces human glutamate oxaloacetate transaminase with the amino acid sequence SEQ ID NO:2, obtained by transformation of strain BL21 (DE3) Rosetta2pLysS with plasmid pET22b containing a cloned sequence GOT1 with ten C-terminal histidines.
EFFECT: invention provides a high level of expression of the target protein in a soluble active form characterized by a high level of enzyme activity.
1 cl, 3 dwg, 1 ex
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