METHOD FOR PRODUCING A BIORESORBED TUBE BASED ON METHACRYLIZED GELATINE AND METHACRYLIZED FIBROIN AND A METHOD FOR INCREASING THE CONSISTENCY OF INTESTINAL ANASTOMOSIS USING SUCH A TUBE Russian patent published in 2020 - IPC A61F2/02 A61F2/04 A61K38/17 A61L27/22 A61F2/82 A61B17/03 A61B17/11 A61K35/64 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology and medicine. In more detail, the invention refers to surgery, namely to methods for reducing the incidence of intestinal anastomosis. Invention relates to a method of producing a bioresorbable tube based on methacrylized gelatine and methacrylized fibroin, involving the following steps: a) dissolving in hexafluoroisopropanol of macromonomers: methacrylized gelatine (GMA) at rate of 10 wt% and methacrylized fibroin (FMA) in amount of 4 wt% at 55±5 °C for 18–36 hours; b) obtaining a mixture which includes equal parts of solutions of GMA and FMA and a photoinitiator of diphenyl(2,4,6-trimethylbenzoyl)phosphine in amount of 5 wt% by weight of macromonomers; c) preparing a guide for forming a tube by degreasing, followed by drying a surface of a glass rod with a circular cross-section of 2–15 cm in diameter; d) preparing the reinforcing thread by holding untwisted 8/0-4/0 silk thread in the mixture obtained in step b); e) forming the first layers of the bioresorbable tube on the guide by immersing it in the mixture obtained at step b) for 5–10 seconds with subsequent rotation and exposure of the obtained workpiece in the ultraviolet lamp light for at least 2 minutes; f) reinforcing the end sections of the tube by laying the thread in turns at distance of 2–5 mm from the ends of the formed tube to the workpiece obtained at step e); g) forming the outer layers of the bioresorbable tube by immersing the workpiece obtained at step f) into the mixture obtained at step b) for 5–10 seconds, followed by rotating and exposing the obtained tube in the ultraviolet lamp light for at least 2 minutes; h) processing the formed tube by holding in distilled water for 1–1.5 hours, followed by holding in 96 % ethanol for 12–18 hours and repeated holding in distilled water for 1–1.5 hours, treating internal surface of the formed tube with chloroform.

EFFECT: obtaining articles having a programmable rate of degradation, low immunogenicity, ease of modification; developing a new approach to solving the problem of anastomosis inconsistency, where, in addition to mechanical protection of the anastomosis area, the implant is actively built into the regeneration process.

11 cl, 3 dwg, 2 ex