METHOD OF PRODUCING POLYBROMOLINE PREPARATION USING GLUTARIC ALDEHYDE Russian patent published in 2020 - IPC C12N11/04 C12N11/10 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology. Method of producing polybromoline preparation involves copolymerization using glutaraldehyde as a cross-linking agent, which is carried out by dissolving bromelain in a tris-glycine buffer solution with pH 9.0 from 1 mg of bromelaine per 1 ml of a buffer solution, adding glutaric aldehyde solution with concentration of 2 % or 10 % in volume equal to volume of tris-glycine buffer solution, incubating to formation of film, washing with 50 mM of Tris-HCl buffer solution (pH 7.5) to absence in washing water protein.

EFFECT: invention allows to increase the rate of enzymatic reaction and to increase the effectiveness of using the preparation based on bromelain at high temperatures.

1 cl, 1 dwg, 1 ex