FIELD: biotechnology.
SUBSTANCE: method for producing a composite preparation of bromelain and sodium alginate in the form of a thick solution is proposed, including the immobilization of the enzyme preparation of bromelain in a thick solution of sodium alginate in the ratio of 10 ml of a bromelain solution at a concentration of 20 mg/ml per 1 g of dry sodium alginate, previously dissolved in 10 ml of buffer, incubation at room temperature for 2 hours with constant stirring at a speed of 250 rpm; 0.05 M glycine buffer with pH 8.6 containing cysteine at a concentration of 0.04 M is used as a buffer solution for immobilization. The resulting preparation is washed by dialysis with 50 mM Tris-HCl buffer with pH 7.5.
EFFECT: invention provides an extension of the arsenal of methods for obtaining stabilized bromelain preparations used in medical practice.
1 cl, 3 dwg, 1 ex
Title | Year | Author | Number |
---|---|---|---|
METHOD OF PRODUCING A HYBRID PREPARATION OF BROMELAIN AND CHITOSAN ASCORBATE IN FORM OF A THICK SOLUTION | 2023 |
|
RU2819793C1 |
METHOD FOR PRODUCING HYBRID PREPARATION OF BROMELAIN AND CARBOXYMETHYLCELLULOSE IN THE FORM OF A DENSE SOLUTION | 2022 |
|
RU2788454C1 |
METHOD FOR OBTAINING A COMPOSITE PREPARATION OF PAPAIN AND SODIUM ALGINATE IN THE FORM OF A THICK SOLUTION | 2022 |
|
RU2788455C1 |
METHOD OF PRODUCING HYBRID PREPARATION OF PAPAIN AND CHITOSAN ASCORBATE IN FORM OF THICK SOLUTION | 2023 |
|
RU2822736C1 |
METHOD FOR PRODUCING A HETEROGENEOUS BROMELINE PREPARATION WHICH IS COVALENTLY BOUND TO A CHITOSAN MATRIX | 2018 |
|
RU2711786C1 |
METHOD OF OBTAINING A HETEROGENEOUS DRUG BASED ON COLLAGENASE AND CHITOSAN | 2017 |
|
RU2678435C2 |
METHOD FOR OBTAINING HETEROGENEOUS PREPARATION OF VARIOUS DISPERSITIES BASED ON BROMELAIN AND CHITOSAN | 2017 |
|
RU2677232C2 |
METHOD OF PRODUCING BROMELAIN IN GEL BASED ON FOOD CHITOSAN AND CHITOSAN SUCCINATE | 2018 |
|
RU2691611C1 |
METHOD FOR OBTAINING FICIN AND CHITOSAN ACETATE HYBRID PREPARATION IN THE FORM OF A DENSE SOLUTION | 2022 |
|
RU2792783C1 |
METHOD FOR OBTAINING HYBRID PREPARATION OF PAPAIN AND CARBOXYMETHYLCELLULOSE IN THE FORM OF A DENSE SOLUTION | 2022 |
|
RU2795425C1 |
Authors
Dates
2023-03-24—Published
2022-08-01—Filed